Double-label immunofluorescence method for simultaneous detection of adenovirus and herpes simplex virus from the eye
Double-label immunofluorescence method for simultaneous detection of adenovirus and herpes simplex virus from the eye
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双标记免疫荧光法同时检测眼内腺病毒和单纯疱疹病毒
DOI：
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发表时间：
1989
影响因子：
9.4
通讯作者：
S. Darougar
中科院分区：
文献类型：
--
作者：
P. Walpita;S. Darougar
描述了双标记免疫荧光方法的开发和应用，该方法有可能在单壳小瓶培养物中筛选来自任何位点的单一或双重感染。在本研究中，总共1,141个眼部标本接种于贝壳瓶中，15,000 X g离心1小时，37℃孵育48小时，并在室温下于甲醇中固定15分钟。通过双标记间接免疫荧光程序染色来检测病毒包涵体，其中使用适当的第一抗体的混合物，然后使用荧光素和罗丹明缀合的第二抗体。每个标本也通过常规病毒分离方法平行接种。双标壳小瓶程序的灵敏度和特异性与传统方法相当，并且前者测试仅需48小时即可完成。该测试提供了快速而简单的单瓶程序，可以单独或同时检测多种病原体。与传统的病毒分离方法和其他壳瓶程序相比，它可以节省时间和成本。
The development and application of a double-label immunofluorescence method which has the potential to screen for single or dual infections from any site, in single shell vial cultures, is described. In this study, a total of 1,141 ocular specimens were inoculated in shell vials, centrifuged at 15,000 X g for 1 h, incubated at 37 degrees C for 48 h, and fixed in methanol at room temperature for 15 min. The virus inclusions were detected by staining with a double-label indirect immunofluorescence procedure using mixtures of appropriate first antibodies, followed by fluorescein- and rhodamine-conjugated second antibodies. Each specimen was also inoculated in parallel by the conventional virus isolation method. The sensitivity and specificity of the double-label shell vial procedure were comparable to those with the conventional method, and the former test took only 48 h to complete. The test offers a rapid and simple single-vial procedure which allows for individual or simultaneous detection of multiple pathogens. It results in savings in time and cost over the conventional virus isolation method and other shell vial procedures.
一种使用相同同种型抗体通过间接程序进行双重免疫荧光染色的方法。
影响因子：
2.2
作者：
Bologna,M;Allen,R;Dulbecco,R
通讯作者：
Dulbecco,R