Trichoderma asperellum strains confer tomato protection and induce its defense-related genes against the Fusarium wilt pathogen
Trichoderma asperellum strains confer tomato protection and induce its defense-related genes against the Fusarium wilt pathogen
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DOI：
10.1007/s40858-016-0098-0
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发表时间：
2016-10-01
影响因子：
2.5
通讯作者：
Molan, Younes Y.
中科院分区：
文献类型：
--
作者：
El Komy, Mahmoud H.;Saleh, Amgad A.;Molan, Younes Y.
番茄枯萎病（Fusarium oxysporum f. sp. lycopersici， FOL）引起的番茄枯萎病（Fusarium wilt of tomato， FW）是全球番茄生产面临的主要挑战。在温室条件下，对5株曲霉的生长潜力进行了评价。结果表明，经曲霉菌株处理后，与仅经foll处理的植株相比，foll侵染植株的发病率和严重程度均显著降低。在曲霉菌株处理的植株上，枯萎病的减少伴随着番茄茎和根际FOL种群的显著减少。此外，无论是否存在FOL，曲霉的施用都能促进番茄植株的生长。选择两株在减少病害发展和提高植株生长参数方面表现最好的曲霉菌株TS-12和TS-39，以阐明其触发番茄防御机制的能力。防御相关基因、几丁质酶（SlChi3）、β -1,3-葡聚糖酶（SlGluA）和PR-1 （SlPR-1a）在木霉处理、FOL感染植株的茎和根中的表达水平与仅感染FOL的植株相比显著升高。这些结果表明，利用曲霉菌株TS-12和TS-39的拮抗活性和诱导系统抗性的能力，可以作为控制FW的替代策略。
Fusarium wilt of tomato (FW) caused by Fusarium oxysporum f. sp. lycopersici (FOL) is a major challenge for tomato production worldwide. For sustainable management of FW, the potential of five strains of Trichoderma asperellum was evaluated under greenhouse conditions. The results indicated that FOL infected plants treated with T. asperellum strains significantly reduced disease incidence and severity compared with FOL-only infected plants. The reduction of wilt disease on plants treated with T. asperellum strains was accompanied by a significant reduction in FOL populations in tomato stems and rhizosphere. Moreover, the application of T. asperellum promoted tomato plant growth irrespective of the presence or absence of FOL. Two strains of T. asperellum (TS-12 and TS-39) that showed the best performance in minimizing disease development and increases in plant growth parameters were selected for elucidating their ability in triggering tomato defense mechanisms. The expression levels of defense-related genes, chitinase (SlChi3), beta-1,3-glucanase (SlGluA) and PR-1 (SlPR-1a) were significantly increased in the stems and roots of Trichoderma treated, FOL infected plants, compared with FOL-only infected ones. These results indicate that the application of T. asperellum strains TS-12 and TS-39 can be used as an alternative strategy to manage FW through their antagonistic activities and abilities to induce systemic resistance.