Developing an articular cartilage decellularization process toward facet joint cartilage replacement.
Developing an articular cartilage decellularization process toward facet joint cartilage replacement.
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DOI：
10.1227/01.neu.0000367616.49291.9f
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发表时间：
2010-04
期刊：
影响因子：
4.8
通讯作者：
Athanasiou KA
中科院分区：
文献类型：
--
作者：
Elder BD;Kim DH;Athanasiou KA
组织工程似乎是一个有前途的战略，关节软骨再生作为一种治疗小关节关节炎。在开始组织工程方法之前，必须建立设计标准以确定替代组织所需的功能特性。由于小关节软骨的功能特性的表征以前没有进行过，本研究的目的是确定小关节软骨的生物力学，生物化学和组织学特性。使用从脊椎成熟犬获得的四个腰椎节段进行体外试验。在每个标本中，从L3-L4和L4-L5小关节的上级表面获得关节软骨。蠕变压痕用于确定压缩生物力学性能，而单轴拉伸试验产生的杨氏模量和极限拉伸强度的组织。此外，生化评估包括细胞结构、GAG含量和胶原蛋白含量的测定，以及胶原蛋白I和II产生的ELISA。最后，组织学表征包括H&E染色以及胶原和GAG分布的染色。测定平均值±标准差。两个级别之间的任何评估属性均无差异。两个节段的平均厚度为0.49±0.10 mm，水合率为74.7± 1.7%。此外，细胞/WW比率为6.26±2.66 × 104个细胞/mg，细胞/DW比率为2.51±1.21 × 105个细胞/mg。GAG/WW为0.038±0.013，GAG/DW为0.149±0.049 mg/mg;胶原/WW为0.168±0.026，胶原/DW为0.681±0.154 mg/mg。最终，聚集体模量为554±133 kPa，杨氏模量为10.08±8.07 MPa，极限拉伸强度为4.44±2.40 MPa。据我们所知，这项研究是第一个提供了小关节关节软骨的功能特性，从而为未来的组织工程研究提供了设计标准。
Tissue engineering appears to be a promising strategy for articular cartilage regeneration as a treatment for facet joint arthritis. Prior to the commencement of tissue engineering approaches, design criteria must be established to determine the required functional properties of the replacement tissue. As characterization of functional properties of facet joint cartilage has not been performed previously, the objective of this study was to determine the biomechanical, biochemical, and histological properties of facet joint cartilage. The in vitro testing was conducted using four lumbar spinal segments obtained from skeletally mature canines. In each specimen, articular cartilage was obtained from the superior surface of the L3–L4 and L4–L5 facet joints. Creep indentation was used to determine the compressive biomechanical properties, while uniaxial tensile testing yielded the Young’s modulus and ultimate tensile strength of the tissue. Additionally, biochemical assessments included determinations of cellularity, GAG content, and collagen content, as well as ELISAs for collagen I and II production. Finally, histological characterization included H&E staining, as well as staining for collagen and GAG distributions. The mean ± standard deviation values were determined. There were no differences between the two levels for any of the assessed properties. Averaged over both levels, the thickness was 0.49±0.10 mm and the hydration was 74.7±1.7%. Additionally, the cells/WW ratio was 6.26±2.66 × 104 cells/mg and the cells/DW ratio was 2.51±1.21 × 105 cells/mg. The GAG/WW was 0.038±0.013 and the GAG/DW was 0.149±0.049 mg/mg, while the collagen/WW was 0.168±0.026 and collagen/DW was 0.681±0.154 mg/mg. Finally, the aggregate modulus was 554±133 kPa, the Young’s modulus was 10.08±8.07 MPa, and the ultimate tensile strength was 4.44±2.40 MPa. To the best of our knowledge, this study is the first to provide a functional characterization of facet joint articular cartilage, thus providing design criteria for future tissue engineering studies.