LEAFHOPPER TRANSMISSION OF A VIRUS CAUSING MAIZE WALLABY EAR DISEASE
LEAFHOPPER TRANSMISSION OF A VIRUS CAUSING MAIZE WALLABY EAR DISEASE
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DOI：
10.1111/j.1744-7348.1975.tb01584.x
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发表时间：
1975-01-01
影响因子：
2.6
通讯作者：
GRYLLS, NE
中科院分区：
文献类型：
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作者：
GRYLLS, NE
用双斑蝉（Cicadulina bimaculatata）对14种单子叶植物进行了传毒试验。也可通过苍白新蚤（Nesoclutha pallida）和嫁接传播。所获得的症状与菲律宾的玉米叶瘿病和意大利和以色列的玉米粗缩病毒报道的症状非常相似。在田间捕获的双斑携带玉米沙袋鼠耳病毒（MWEV），并且它们的许多后代即使在不允许接近受感染的植物时也是带病毒的。在从选定的非传播性成虫繁殖的9代克隆中，感染性个体的比例从第一代的85%下降到不到1%;这样的个体很难饲养，因为它们的繁殖力和寿命大大下降。pallidata感染MWEV后，注射感染植物的部分纯化提取物。双斑的，但不是在那些非传播的个人。颗粒发生在细胞质中的小管中，每个都有一个密集染色的核心。直径为50 nm。在感染但未感染的玉米提取物中，以及在含病毒但未含病毒的玉米提取物中，发现了与核心大小相似的颗粒。bimaculataandN.苍白球
A virus causing maize wallaby ear disease was transmitted experimentally byCicadulina bimaculatato fourteen species of monocotyledonous plants. It was also transmitted byNesoclutha pallida, and by grafting. The symptoms obtained resemble closely those reported for maize leaf gall disease in the Philippines and maize rough dwarf virus in Italy and Israel.About 85% ofC. bimaculatacaught in the field carried maize wallaby ear virus (MWEV), and many of their progeny were viruliferous even when not allowed access to infected plants. The proportion of infective individuals in clones bred for nine generations from selected non‐transmitting adults decreased from 85% in the first nymphs to less than 1%; such individuals were difficult to rear, as their fecundity and longevity decreased greatly.N. pallidatransmitted MWEV after injection with partially purified extracts of infected plants.Spherical particlesc.85 nm in diameter were found in the salivary glands of viruliferousC. bimaculata, but not in those of non‐transmitting individuals. The particles occurred in tubules in the cytoplasm and each had a densely stained corec.50 nm in diameter. Particles similar in size to the core were found in extracts of infected but not uninfected maize, and in extracts of viruliferous but not in non‐viruliferousC. bimaculataandN. pallida.