A 29-kDa merozoite protein is a prospective antigen for serological diagnosis of Babesia orientalis infection in buffaloes.
A 29-kDa merozoite protein is a prospective antigen for serological diagnosis of Babesia orientalis infection in buffaloes.
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29 kDa 裂殖子蛋白是水牛东方巴贝虫感染血清学诊断的前瞻性抗原。
DOI：
10.1016/j.vetpar.2009.03.001
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发表时间：
2009
影响因子：
2.6
通讯作者：
Junlong Zhao
中科院分区：
文献类型：
--
作者：
Dan Zhou;Fen Du;Qin Liu;Qin Liu;Yanqin Zhou;Junlong Zhao
通过对B的免疫筛选，分离到编码东方Babebacteriumorientalis（BoP 29）的29-kDa蛋白的基因。结果表明，该cDNA序列为971 bp，开放阅读框为750 bp。NCBI中的BLASTn和BLASTx分析结果表明，该cDNA序列与牛巴氏杆菌假想蛋白（BBOV_II005480）具有相似性。Western blotting结果显示重组蛋白rBoP 29与B呈阳性反应。东方蜱感染布法罗血清。以rBoP 29为抗原建立了间接酶联免疫吸附试验（ELISA）。该方法可检测实验感染B的水牛血清中特异性抗体的变化。东方对132份水牛血清的检测结果表明，rBoP 29-ELISA和PCR检测阳性样本的相对有效性差异不显著（χ2=0.910; P=0.897）。这些结果表明BoP 29是B的新抗原。rBoP 29可能是诊断B的良好候选抗原。布法罗东方蜱感染
A gene encoding a 29-kDa protein of Babesia orientalis (BoP29) was isolated by immunoscreening the B. orientalis cDNA expression library using monoclonal antibodies and its nucleotide sequence of the cDNA was 971bp with an open reading frame of 750bp. The results of BLASTn and BLASTx in NCBI showed that this cDNA sequence had similarity with Babesia bovis hypothetical protein (BBOV_II005480). Western blotting showed that the recombinant protein (rBoP29) had positive seroreactivity to B. orientalis infected buffalo sera. An indirect enzyme-linked immunosorbent assay (ELISA) was developed using the rBoP29 as antigen. This method could detect specific antibody variation in the sera from the buffaloes experimentally infected with B. orientalis. Analysis of 132 sera samples collected from buffaloes showed that there was no significant difference in relative effectiveness of rBoP29-ELISA and PCR (χ2=0.910; P=0.897) in identifying positive samples. These results indicate that the BoP29 is a novel antigen of B. orientalis, and rBoP29 might be a good antigen candidate for diagnosis of B. orientalis infection in buffalo.