Modulation of peripheral-type benzodiazepine receptor levels in a reperfusion injury pig kidney-graft model.
Modulation of peripheral-type benzodiazepine receptor levels in a reperfusion injury pig kidney-graft model.
复制标题
再灌注损伤猪肾移植模型中外周型苯二氮卓受体水平的调节。
DOI：
10.1097/00007890-200212150-00006
复制
发表时间：
2002
期刊：
影响因子：
6.2
通讯作者：
Papadopoulos,Vassilios
中科院分区：
文献类型：
--
作者：
Hauet,Thierry;Han,Zeqiu;Wang,Yan;Hameury,Frederic;Jayle,Christophe;Gibelin,Helene;Goujon,JeanMichel;Eugene,Michel;Papadopoulos,Vassilios
背景：缺血再灌注损伤与急性排斥反应、移植物功能延迟或慢性移植物功能障碍的风险增加有关。线粒体发挥核心作用，在这个process.Methods。使用自体移植猪肾模型，早期（40分钟和7天）和晚期（2-16周）的肾功能和形态的变化后，确定不同时期的冷缺血在威斯康星州大学或欧洲柯林斯解决方案。我们还研究了外周型苯二氮受体（PBR），这也是在维持外线粒体膜stability.Results的关键的表达。肾脏的功能是更好地保存后1小时和24小时比后48小时和72小时在欧洲柯林斯和威斯康星州大学的解决方案。保存1 h和24 h组的延髓损伤减轻。在正常人和保存良好的猪肾中，PBR均存在于肾皮质和髓质的上皮细胞中。PBR的表达是由缺血-再灌注损伤和并发肾小管损伤和修复processs.Conclusion.This研究表明，PBR的表达与肾脏保存的质量，并可能作为肾脏和线粒体活力的指标。此外，这些数据表明，PBR可能参与再灌注过程中的膜生物合成。此外，考虑到PBR在人和猪肾脏中的相同定位，这些发现可能直接应用于肾脏病理学的人类临床环境。
Background.Ischemia-reperfusion injury is associated with an increased risk of acute rejection, delayed graft function, or chronic graft dysfunction. Mitochondria play a central role in this process.Methods.Using an autotransplantation pig kidney model, both early (40 min and 7 days) and late (2–16 weeks) changes in renal function and morphology were determined after different periods of cold ischemia in University of Wisconsin or Euro-Collins solutions. We have also investigated the expression of the peripheral-type benzodiazepine receptor (PBR), which is also critical in maintaining outer mitochondrial membrane stability.Results.Function of the kidneys was better preserved after 1 hr and 24 hr than after 48 hr and 72 hr in Euro-Collins and University of Wisconsin solutions. Medulla injury was reduced in 1 hr-preserved and 24 hr-preserved groups. PBR was found to be present in epithelial cells of the deep cortical and outer medulla in both normal human and well-preserved pig kidneys. PBR expression was modulated by ischemia-reperfusion injury and the concurrent tubular injury and repair processes.Conclusion.This study indicates that PBR expression correlates with the quality of kidney preservation and might serve as an index of kidney and mitochondria viability. Moreover, these data suggest that PBR might be involved in membrane biogenesis during reperfusion. In addition, considering the identical localization of PBR in human and pig kidneys, these findings could have a direct application in human clinical settings of kidney pathology.