Liposome clearance in mice: the effect of a separate and combined presence of surface charge and polymer coating
Liposome clearance in mice: the effect of a separate and combined presence of surface charge and polymer coating
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DOI：
10.1016/s0378-5173(02)00129-1
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发表时间：
2002-06-20
影响因子：
5.8
通讯作者：
Torchilin, VP
中科院分区：
文献类型：
--
作者：
Levchenko, TS;Rammohan, R;Torchilin, VP
我们的工作的目的是比较不同表面性质的脂质体的生物分布。制备含有6%mol带电脂质（硬脂胺，SA;磷脂酸）的磷脂酰胆碱（PC）/胆固醇（Chol）脂质体。PA：或磷脂酰丝氨酸。PS）和/或不同MW（750和5000）的聚乙二醇（PEG）-PE。研究了小鼠中的zeta电位和脂质体清除率。体外PEG以类似方式的连接中和了带电组分的作用。在体内，带电脂质的化学性质变得重要。短PEG 750和长PEG 5000均抑制带正电荷的SA-脂质体的清除，而仅长PEG 5000抑制带负电荷的PA-脂质体的清除，并且没有PEG抑制带负电荷的PS-脂质体的清除。不同分子大小的调理素可能参与不同电荷脂质体的清除。(C)2002 Elsevier Science B. V.保留所有权利。
The purpose of our work was to compare the biodistribution of liposomes with different surface properties. Phosphatidylcholine (PC)/cholesterol (Chol) liposomes were prepared containing 6% mol of a charged lipid (stearylamine, SA; phosphatidic acid. PA: or phosphatidyl serine. PS) and/or polyethylene glycol (PEG)-PE of different MW (750 and 5000). zeta-Potentials and liposome clearance in mice were investigated. In vitro. the attachment of PEG in a similar fashion neutralizes the effect of,my charged component. In vivo, the chemical nature of a charged lipid becomes important. Both short PEG750 and longer PEG5000 inhibit the clearance of positively charged SA-liposomes, while only longer PEG5000 inhibits the clearance of negatively charged PA-liposomes and none of the PEGs inhibit the clearance of negatively charged PS-liposomes. The opsonins with different molecular size may be involved in the clearance of liposomes containing different charged lipids. (C) 2002 Elsevier Science B.V. All rights reserved.