ISOPRENYLATION OF BRAIN 2',3'-CYCLIC NUCLEOTIDE 3'-PHOSPHODIESTERASE MODULATES CELL MORPHOLOGY
ISOPRENYLATION OF BRAIN 2',3'-CYCLIC NUCLEOTIDE 3'-PHOSPHODIESTERASE MODULATES CELL MORPHOLOGY
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DOI：
10.1002/jnr.490390405
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发表时间：
1994-11-01
影响因子：
4.2
通讯作者：
BRAUN, PE
中科院分区：
文献类型：
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作者：
DEANGELIS, DA;BRAUN, PE
CNP（2',3'-环核苷酸3'-磷酸二酯酶）是由少突胶质细胞（OLs）合成的最早的髓鞘特异性多肽。当非髓鞘“幼稚”细胞转染大鼠CNP cDNA时，CNP以点状方式在细胞内以及质膜上积累。丝状伪足和丝状突，如ol的丝状伪足和丝状突变得细长且数量更多，并充满了这种蛋白质。翻译后末端C-T-I-I序列与法尼基或香叶基的异戊二烯化对这种现象至关重要。相比之下，非异丙烯化的C397S突变体在细胞质中均匀分布，对细胞形态没有明显影响。我们在体外合成了CNP和C397S突变体，并证明异戊二烯化是新合成的CNP与髓磷脂结合的必要条件。(C) 1994 Wiley-Liss, Inc。
CNP (2',3'-cyclic nucleotide 3'-phosphodiesterase) is the earliest myelination specific polypeptide to be synthesized by oligodendrocytes (OLs). When non-myelinating ''naive'' cells are transfected with the rat CNP cDNA, CNP accumulates intracellularly in a punctate manner, as well as at the plasma membrane. Filopodia and processes, like those of OLs become elongated and more numerous, and are filled with this protein. Post-translational isoprenylation of the terminal C-T-I-I sequence with either farnesyl or geranylgeranyl is essential for this phenomenon. In contrast, the non-isoprenylated C397S mutant is homogeneously distributed throughout the cytoplasm and does not markedly affect cellular morphology. We have synthesized CNP and the C397S mutant in vitro and have shown that isoprenylation is essential for the binding of newly synthesized CNP to myelin. (C) 1994 Wiley-Liss, Inc.