Laser light scattering study of polyacrylamide gels
Laser light scattering study of polyacrylamide gels
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聚丙烯酰胺凝胶的激光光散射研究
DOI：
10.1002/polb.1987.090250401
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
D. Sellen
中科院分区：
文献类型：
--
作者：
D. Sellen
描述了用含有扩散牛血浆白蛋白的聚丙烯酰胺凝胶进行的激光散射实验。完整的外差拍没有被假定，并考虑到一种新的方法，寻找扫描角度的散射，同时测量时间自相关函数的光谱展宽的程度。通过这种方法获得的聚丙烯酰胺凝胶和非交联的解决方案中的扩散蛋白的情况下，与其他作者的结果进行了比较。在蛋白质的存在下，在蛋白质的扩散系数和与聚丙烯酰胺的链段密度的扩散波动相关的系数之间存在复杂的相互作用。扩散蛋白在低凝胶浓度下占主导地位，并且凝胶密度波动在高凝胶浓度下占主导地位。有人提出，蛋白质优先占据凝胶的密度较低的区域，因此在低凝胶浓度下，蛋白质的扩散速度快于凝胶波动，因此蛋白质倾向于抵消凝胶波动，而在高凝胶浓度下，蛋白质受到凝胶结构的高度阻碍，因此蛋白质倾向于被凝胶密度波动沿着。
Laser light scattering experiments with polyacrylamide gels containing diffusing bovine plasma albumin are described. Complete heterodyne beating has not been assumed, and an account is given of a novel method for finding the degree of spectral broadening by scanning angles of scatter while measuring the temporal autocorrelation function. Results obtained by this method for polyacrylamide gels and un-cross-linked solutions in the absence of diffusing protein, are compared with those of other authors. In the presence of the protein there is a complex interaction between the diffusion coefficient of the protein and the coefficient associated with the diffusing fluctuations in segment density of polyacrylamide. The diffusing protein dominates at low gel concentrations, and the gel density fluctuations dominates at high gel concentrations. It is proposed that the protein preferentially occupies the less dense regions of the gel, so that at low gel concentrations, where it diffuses faster than the gel fluctuations, it tends to cancel them out, whereas at high gel concentrations where the protein is highly impeded by the gel structure, it tends to be carried along by the gel density fluctuations.