First detection of a putative knockdown resistance gene in major mosquito vector, Aedes albopictus.
First detection of a putative knockdown resistance gene in major mosquito vector, Aedes albopictus.
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DOI：
10.7883/yoken.64.217
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发表时间：
2011-05
影响因子：
2.2
通讯作者：
S. Kasai;L. Ng;S. Lam-Phua;C. Tang;Kentaro Itokawa;O. Komagata;Mutsuo Kobayashi;T. Tomita
中科院分区：
文献类型：
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作者：
S. Kasai;L. Ng;S. Lam-Phua;C. Tang;Kentaro Itokawa;O. Komagata;Mutsuo Kobayashi;T. Tomita
亚洲虎蚊，白纹伊蚊（Skuse），是基孔肯雅热的主要媒介，也是登革热的次要媒介。我们收集了阿。来自新加坡的白纹伊蚊，并进行基因分型测定以检测电压门控钠通道（拟除虫菊酯杀虫剂的靶位点）的突变。我们检测到一个氨基酸取代，F1534C，这是怀疑赋予击倒抗性（kdr）拟除虫菊酯类杀虫剂。在收集的蚊子中，53.8%为该突变的纯合型，该突变的等位基因频率估计为73.1%。结构域II和IV的其他5个位点均未检测到kdr突变。这是第一个在Ae中存在kdr基因的证据。白纹伊蚊，我们的研究结果强调需要研究全球分布的等位基因在这个重要的媒介昆虫。
The Asian tiger mosquito, Aedes albopictus (Skuse), is the major vector of Chikungunya fever and the secondary vector of dengue fever. We collected Ae. albopictus from Singapore and performed genotyping assay to detect mutations of the voltage-gated sodium channel, which is the target site of pyrethroid insecticides. We detected an amino acid substitution, F1534C, which is suspected to confer knockdown resistance (kdr) to pyrethroid insecticides. Of the collected mosquitoes, 53.8% were homozygous for this mutation, and the allele frequency of this mutation was estimated to be 73.1%. No kdr mutation was detected in the 5 other loci of domains II and IV. This is the first evidence for the presence of the kdr gene in Ae. albopictus, and our findings highlight the need for studying the global distribution of this allele in this important vector insect.