Synchrotron FTIR Microspectroscopy Study of the Striatum in 6-Hydroxydopamine Rat Model of Parkinson's Disease
Synchrotron FTIR Microspectroscopy Study of the Striatum in 6-Hydroxydopamine Rat Model of Parkinson's Disease
复制标题
6-羟基多巴胺帕金森病大鼠模型纹状体的同步加速器 FTIR 显微光谱研究
DOI：
10.1155/2012/176937
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发表时间：
2012-06
期刊：
影响因子：
--
通讯作者：
Wang Yuyin
中科院分区：
文献类型：
--
作者：
Zhu Hongyan;Xiao, Pei;Wu Lingyan;Bo, Liu;Qi Zeming;Wang Yuyin
本研究采用同步加速器傅立叶变换-红外（FTIR）显微光谱技术分析了正常和帕金森病大鼠脑组织纹状体神经元的生化组成。用6-羟多巴胺（6-OHDA）破坏黑质纹状体通路，建立帕金森病大鼠模型。详细的光谱分析显示，与对照组相比，6- ohda损伤的PD大鼠纹状体神经元中脂质和蛋白质等细胞成分发生了显著变化。结果，PD大鼠纹状体神经元脂质光谱吸收强度高于对照动物。此外，PD神经元中磷脂的不饱和水平相对于对照神经元降低，表明脂质过氧化水平较高。蛋白质二级结构分析表明？与对照组相比，PD神经元的-sheet出现了异常，表明纹状体神经元的异常蛋白结构在其形态出现之前就发生了。这些发现提示神经元的生化变化可能参与了帕金森病的发病机制。
In the present study, synchrotron-based Fourier transform-infrared (FTIR) microspectroscopy is used to analyze the biochemical composition of the striatal neurons in normal and Parkinson's disease (PD) rat brain tissues. The rat model of Parkinson's disease is established by destroying the nigrostriatal pathway with 6-hydroxydopamine (6-OHDA). The detailed spectral analyses show the significant changes of cellular compositions such as lipids, and proteins in the striatal neurons of 6-OHDA-lesioned PD rats with respect to control neurons. As a result, the intensities of spectral absorption assigned to lipid of the striatal neurons in PD rats are higher than in control animals. Furthermore, the unsaturation levels of phospholipids decrease in PD neurons with respect to control neurons, indicating a high level of lipid peroxidation. The analysis of protein secondary structure shows the significantly higher ratio of ?-sheet in PD neurons compared to that of control neurons, suggesting that the abnormal protein structure occurs before their morphological appearances in the striatal neurons. These findings suggest that the biochemical changes in neurons could be involved in the pathogenesis of Parkinson's disease.
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DOI：
--
发表时间：
2002
期刊：
Nature reviews. Neuroscience
影响因子：
--
作者：
J. Lotharius;P. Brundin
通讯作者：
J. Lotharius;P. Brundin
DOI：
10.1016/s0925-4439(97)00070-7
发表时间：
1997-11-28
影响因子：
6.2
作者：
Cassarino, DS;Fall, CP;Bennett, JP
通讯作者：
Bennett, JP
影响因子：
1.9
作者：
DUNCAN, WD;WILLIAMS, GP
通讯作者：
WILLIAMS, GP
影响因子：
2.5
作者：
Miller, LM;Smith, RJ
通讯作者：
Smith, RJ
影响因子：
5.2
作者：
Zhou C;Huang Y;Przedborski S
通讯作者：
Przedborski S