Structural and functional interactions between extraradical mycelia of ectomycorrhizal Pisolithus isolates
Structural and functional interactions between extraradical mycelia of ectomycorrhizal Pisolithus isolates
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外生菌根豆毛菌分离株的根外菌丝体之间的结构和功能相互作用
DOI：
10.1111/j.1469-8137.2012.04126.x
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发表时间：
2012
期刊：
影响因子：
9.4
通讯作者：
Bingyun Wu
中科院分区：
文献类型：
--
作者：
Morishige;T.;Tamakoshi;M.;Takemura;T.;Sato;F.;Bingyun Wu
·来自不同外生菌根（ECM）根的根外菌丝体在森林地面下共存并相互作用。我们研究了同种菌丝体的结构联系以及相同或不同基因间碳和磷的转运。将相同或不同Pisolithuisolates定殖的成对ECMPinus thunbergi幼苗在根箱中并排生长，因为它们的菌丝彼此接触。将14 CO2或33 P-磷酸进料到成对幼苗之一的叶片或菌丝上的斑点。使用数字放射自显影技术和成像板显示14 C和33 P的时间过程分布。同一菌株的菌丝在接触点附近有菌丝连接，不同菌株之间没有菌丝连接，14 C和33 P在同一菌株的菌丝之间发生了转移。在33 P-喂养菌丝体，积累的33 P从喂养点向主机ECM根观察。不同菌株菌丝体间存在No14 C和33 P的易位。这些结果提供了直接的证据，即同一ECM分离物的菌丝体之间的接触和菌丝连接可以引起营养物质移位。讨论了根外菌丝接触的生态学意义。
•Extraradical mycelia from different ectomycorrhizal (ECM) roots coexist and interact under the forest floor. We investigated structural connections of conspecific mycelia and translocation of carbon and phosphorus between the same or different genets.•Paired ECMPinus thunbergiiseedlings colonized by the same or differentPisolithusisolates were grown side by side in a rhizobox as their mycelia contacted each other.14CO2or33P‐phosphoric acid was fed to leaves or a spot on the mycelium in one of the paired seedlings. Time‐course distributions of14C and33P were visualized using a digital autoradiographic technique with imaging plates.•Hyphal connections were observed between mycelia of the samePisolithusisolate near the contact site, but hyphae did not connect between different isolates.14C and33P were translocated between mycelia of the same isolate. In33P‐fed mycelia, accumulation of33P from the feeding spot toward the host ECM roots was observed. No14C and33P translocation occurred between mycelia of different isolates.•These results provide direct evidence that contact and hyphal connection between mycelia of the same ECM isolate can cause nutrient translocation. The ecological significance of contact between extraradical mycelia is discussed.