Molecular characterization of a porcine Group A rotavirus strain with G12 genotype specificity
Molecular characterization of a porcine Group A rotavirus strain with G12 genotype specificity
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DOI：
10.1007/s00705-005-0714-7
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发表时间：
2006-07-01
影响因子：
2.7
通讯作者：
Naik, T. N.
中科院分区：
文献类型：
--
作者：
Ghosh, S.;Varghese, V.;Naik, T. N.
在印度加尔各答郊区一个养猪场进行的轮状病毒感染监测研究中，发现了一株猪A组轮状病毒株(RU172)，并对其进行了分子特征分析。在添加G12型特异性引物(本实验室设计用于从现场样品中提取G12分离物)后，通过基于PCR的基因分型分析揭示了RU172的G12基因型特异性，并通过VP7编码基因的序列分析证实了该特异性。在推导的氨基酸水平上，RU172株与人G12株VP7的同源性最高为93.6%~94.5%。尽管RU172具有G12基因型性，但它似乎与人类G12轮状病毒不同，在系统发育分析上，RU172与人类G12株形成了单独的谱系。在所分析的其他基因片段中，RU172属于NSP4B型，具有猪来源的NSP5和VP6，并且与猪P[7]轮状病毒的VP4同源性最高(在推导的氨基酸水平上，VP4的同源性为94.3%-95.4%)。因此，据我们所知，这是第一次检测到具有G12基因特异性的动物轮状病毒株。对RU172等毒株的检测为人类和动物A组轮状病毒的基因组多样性提供了至关重要的见解。
A porcine Group A rotavirus strain (RU172) was detected and molecularly characterized during a surveillance study conducted for rotavirus infection in a pig farm located in a suburban area of Kolkata City, India. The G12 genotype specificity of RU172 was revealed by PCR-based genotyping assays following addition of a G12 type-specific primer (designed in our laboratory to pick up G12 isolates from field samples) and was confirmed by sequence analysis of the VP7-encoding gene. The RU172 strain exhibited maximum VP7 identities of 93.6% to 94.5% with human G12 strains at the deduced amino acid level. In spite of its G12 genotype nature, RU172 appeared to be distinct from human G12 rotaviruses and, on phylogenetic analysis, formed a separate lineage with human G12 strains. Among the other gene segments analyzed, RU172 belonged to NSP4 genotype B, had a NSP5 and VP6 of porcine origin, and shared maximum VP4 identities with porcine P[7] rotaviruses (94.3%-95.4% at the deduced amino acid level). Therefore, to the best of our knowledge, this is the first report of detection of an animal rotavirus strain with G12 genotype specificity. Detection of strains like RU172 provides vital insights into the genomic diversity of Group A rotaviruses of man and animals.