Distinct heat shock factors and chromatin modifications mediate the organ-autonomous transcriptional memory of heat stress
Distinct heat shock factors and chromatin modifications mediate the organ-autonomous transcriptional memory of heat stress
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DOI：
10.1111/tpj.13958
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发表时间：
2018-08-01
期刊：
影响因子：
7.2
通讯作者：
Baeurle, Isabel
中科院分区：
文献类型：
--
作者：
Liu, Hsiang-chin;Laemke, Joern;Baeurle, Isabel
植物可以通过应激信号来启动，以在随后的应激下更快或更强烈地激活防御机制。这种应激启动的一个重要组成部分是在反复出现应激时改变基因的重新激活。然而，人们对其根本机制知之甚少。在这里，我们报告了数十个拟南芥基因表现出转录记忆，即在持续至少 3 天的反复热应激后出现更强的上调。我们定义了一组参与这种记忆反应的转录因子，并表明转录记忆会在热应激提示出现后几分钟内导致转录激活增强。此外，我们表明转录记忆在所有组织中都是活跃的。它可能持续长达一周，并且在此期间与组蛋白 H3 赖氨酸 4 超甲基化有关。这种转录记忆是顺式编码的，因为我们鉴定了一个将记忆赋予异源基因的启动子片段。总之，热诱导的转录记忆是一种广泛且持续的反应，我们的研究为未来高等植物体细胞应激记忆的机制研究提供了框架。
Plants can be primed by a stress cue to mount a faster or stronger activation of defense mechanisms upon subsequent stress. A crucial component of such stress priming is the modified reactivation of genes upon recurring stress; however, the underlying mechanisms of this are poorly understood. Here, we report that dozens of Arabidopsis thaliana genes display transcriptional memory, i.e. stronger upregulation after a recurring heat stress, that lasts for at least 3 days. We define a set of transcription factors involved in this memory response and show that the transcriptional memory results in enhanced transcriptional activation within minutes of the onset of a heat stress cue. Further, we show that the transcriptional memory is active in all tissues. It may last for up to a week, and is associated during this time with histone H3 lysine 4 hypermethylation. This transcriptional memory is cis-encoded, as we identify a promoter fragment that confers memory onto a heterologous gene. In summary, heat-induced transcriptional memory is a widespread and sustained response, and our study provides a framework for future mechanistic studies of somatic stress memory in higher plants.