Functional behavior of chitosan/gelatin/silica-gentamicin coatings by electrophoretic deposition on surgical grade stainless steel
Functional behavior of chitosan/gelatin/silica-gentamicin coatings by electrophoretic deposition on surgical grade stainless steel
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DOI：
10.1016/j.msec.2020.111062
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发表时间：
2020-10-01
影响因子：
7.9
通讯作者：
Ballarre, Josefina
中科院分区：
文献类型：
--
作者：
Aydemir, Tuba;Liverani, Liliana;Ballarre, Josefina
金属在几种骨科应用中用作骨折稳定的固定元件或假体。许多发展中国家最常见的骨科生物材料之一是手术级不锈钢（SS）。然而，由于其在生理介质中的有限耐腐蚀性、缺乏骨整合以及缺乏抗菌作用，其在骨科手术中作为永久性植入物的使用受到限制。这项工作的目的是产生一种可降解的涂层，具有抗菌性能的不锈钢用于植入物/医疗器械。涂层由生物聚合物/二氧化硅-庆大霉素纳米颗粒复合材料组成，通过电泳沉积（EPD）在外科级不锈钢板上获得。通过显微镜检查对涂层表面进行表征，并在磷酸盐缓冲盐水（PBS）溶液、模拟体液（SBF）和细胞培养基中浸泡后评价体外性能，以分析涂层降解、抗生素释放、细胞附着（ST-2基质细胞）和抗菌（大肠杆菌和金黄色葡萄球菌）特性。电泳涂层是均匀的，并均匀地覆盖在SS基板的表面。此外，二氧化硅-庆大霉素纳米颗粒在涂层区域上的分布是均匀的。浸泡7天后，壳聚糖-明胶涂层的降解是明显的。庆大霉素的释放在24 h时导致了优异的抗菌行为，同时细胞增殖（在培养7 d时）未受到抑制。结果表明，涂层系统表现出有前途的行为，这可能有助于防止医院感染的早期植入时间。
Metals are used in several orthopedic applications as fixation elements for the stabilization of fractures or as prostheses. One of the most common orthopedic biomaterials in many developing countries is surgical grade stainless steel (SS). However, its use as permanent implant in orthopedic surgery is conditioned due to its limited corrosion resistance in physiological media, lack of osseointegration, and absence of antibacterial effect. The aim of this work is to generate a degradable coating with antibacterial properties for stainless steel to be used in implants/medical devices. The coating is composed of a biopolymer/silica-gentamicin nanoparticles composite obtained by electrophoretic deposition (EPD) on surgical grade stainless steel plates. The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties. EPD coatings were uniform and covered homogeneously the surface of the SS substrate. Also the distribution of silica-gentamicin nanoparticles was homogeneous on the coated area. The degradation of the chitosan-gelatin coatings was evident after 7 days of immersion. The gentamicin release led to excellent antibacterial behavior at 24 h, meanwhile the cell proliferation (at 7 days culture) was not inhibited. The results show that the coating system exhibits promising behavior which could contribute to prevent hospital infections at early implantation times.