MUCOSAL REACTIVE OXYGEN SPECIES PRODUCTION IN ESOPHAGITIS AND BARRETTS-ESOPHAGUS
MUCOSAL REACTIVE OXYGEN SPECIES PRODUCTION IN ESOPHAGITIS AND BARRETTS-ESOPHAGUS
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DOI：
10.1136/gut.37.2.168
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发表时间：
1995-08-01
期刊：
影响因子：
24.5
通讯作者：
KESHAVARZIAN, A
中科院分区：
文献类型：
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作者：
OLYAEE, M;SONTAG, S;KESHAVARZIAN, A
炎症细胞产生的活性氧（ROS）可导致组织破坏。活性氧与多种胃肠道异常有关，包括与酸相关的消化性疾病。虽然食管炎和巴雷特柱状上皮的发展与胃酸的长时间反流有关，但组织损伤发生的确切机制尚不清楚。为了发现ROS是否参与食管黏膜损伤，本研究采用鲁米诺化学发光法（LECL）体外测量了反流性食管炎患者活检粘膜样本的粘膜ROS浓度。83例患者取活检标本：食管黏膜正常19例（I组）；肉眼可见食管炎20例（II组）；活检证实Barrett上皮，无肉眼食管炎（III组）；Barrett上皮伴肉眼可见食管炎24例（IV组）。患者粘膜的LECL值明显高于对照组。但是，II组、III组和IV组之间没有显著差异。向组织悬浮液中添加髓过氧化物酶抑制剂氮化酶或过氧化氢清除剂过氧化氢酶分别导致LECL值下降32%和45%，这表明中性粒细胞虽然很重要，但并不是粘膜LECL的唯一来源。这些数据与活性氧在食管炎和Barrett柱状上皮相关的组织损伤中起重要作用的建议一致。
Reactive oxygen species (ROS) produced by inflammatory cells can contribute to tissue destruction. ROS have been implicated in various gastrointestinal abnormalities, including the acid related peptic diseases. Although the development of oesophagitis and Barrett's columnar epithelium is associated with prolonged reflux of gastric acid, the exact mechanism by which tissue damage occurs is not known. To discover if ROS are involved in damage to the oesophageal mucosa, this study measured in vitro the mucosal ROS concentrations of biopsied mucosal samples taken from patients with reflux oesophagitis using luminol chemiluminescence (LECL). biopsy specimens were taken from 83 patients: 19 with normal oesophageal mucosa (group I); 20 with macroscopic oesophagitis (group II); 20 with biopsy confirmed Barrett's epithelium without macroscopic oesophagitis (group III); and 24 with Barrett's epithelium with macroscopic oesophagitis (group IV). The mucosa from patients exhibited significantly higher LECL values than the mucosa from controls. But, there were no significant differences between groups II, III, and IV. Addition of the myeloperoxidase inhibitor, azide, or the hydrogen peroxide scavenger, catalase, to the tissue suspension caused a decrease in LECL values of 32% and 45%, respectively, suggesting that neutrophils - although important - are not the only source of mucosal LECL. These data are consistent with the proposal that ROS play an important part in the tissue injury associated with oesophagitis and Barrett's columnar epithelium.