Quantitative Determination of Glycine, Alanine, Aspartic Acid, Glutamic Acid, Phenylalanine, and Tryptophan by Raman Spectroscopy
Quantitative Determination of Glycine, Alanine, Aspartic Acid, Glutamic Acid, Phenylalanine, and Tryptophan by Raman Spectroscopy
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DOI：
10.1080/00032719.2016.1193189
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发表时间：
2017-01-01
影响因子：
2
通讯作者：
Tanaka, Hiroyuki
中科院分区：
文献类型：
--
作者：
Numata, Yasushi;Otsuka, Maria;Tanaka, Hiroyuki
报道了一种利用拉曼光谱对氨基酸进行定量的新方法。测定了甘氨酸、丙氨酸、天冬氨酸、谷氨酸、苯丙氨酸和色氨酸的拉曼光谱。为了消除激光功率强度和仪器效应等因素的影响，计算了氨基酸的拉曼强度与乙腈的拉曼强度之间的谱带比。通过绘制谱带比率与氨基酸浓度的关系图，得到校正曲线。所有氨基酸均呈线性关系，相关系数均在0.99以上。测量了已知浓度样品的拉曼光谱，验证了该方法的重现性。相对误差较小，说明可以用拉曼光谱测定氨基酸的浓度。检出限和定量限分别为背景信号标准偏差的3倍和10倍，分别为0.007和0.02moL L-1。对天冬氨酸在0.02mol.L-1作用下的拉曼光谱进行了多次测量，不确定度为7%。
The development of a new quantitative method for amino acids using Raman spectroscopy is reported. Raman spectra of glycine, alanine, aspartic acid, glutamic acid, phenylalanine, and tryptophan were measured. The band ratio between the Raman intensity of the amino acid and that of acetonitrile as an external standard was calculated to remove the influence of factors such as laser power intensity and instrumental effects. The calibration curves were obtained by plotting the band ratios against the concentrations of the amino acids. The curves were linear with coefficient correlations of over 0.99 for all amino acids. The Raman spectra of known concentration samples were measured to confirm the reproducibility of this method. The relative errors were small, indicating that the concentrations of amino acids can be determined using Raman spectroscopy. The limits of detection and quantitation were determined as thrice and 10 times the standard deviation of the background signal to be 0.007 and 0.02 mol L-1, respectively. Raman spectra of aspartic acid at 0.02 mol L-1 were measured several times and the uncertainty was 7%.