Microscopy with self-reconstructing beams
Microscopy with self-reconstructing beams
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DOI：
10.1038/nphoton.2010.204
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发表时间：
2010-11-01
期刊：
影响因子：
35
通讯作者：
Rohrbach, Alexander
中科院分区：
文献类型：
--
作者：
Fahrbach, Florian O.;Simon, Philipp;Rohrbach, Alexander
虽然自重构光束在过去十年中一直是许多科学研究的焦点，但几乎没有任何关于其在三维非均匀介质中的传播和自愈行为的信息。散射和光束扩散的受控减少将为光学显微镜提供新的照明概念，特别是那些旨在深入观察散射组织的光学显微镜。通过研究三种不同类型的折射率不均匀性，分别使用两个大的玻璃球，一组较小的球体和一块人的皮肤，我们表明，光束自重构确实是可能的。我们表明，贝塞尔光束是出乎意料的强大的对物体的偏转，我们定义的措施，在这种情况下，自我重建。我们提出了一个原型的显微镜与自重建光束（MISERB），并表明，全息形状，扫描贝塞尔光束不仅减少了散射文物，但也同时增加了图像质量和穿透深度在致密介质。
Although self-reconstructing beams have been the focus of many scientific studies over the past decade, hardly anything is known about their propagation and self-healing behaviour in a three-dimensional, inhomogeneous medium. The controlled reduction of scattering and beam spreading would enable a new illumination concept for light microscopes, particularly for those designed to look deep into scattering tissue. By investigating three different classes of refractive index inhomogeneity, using two large glass spheres, a cluster of smaller spheres and a piece of human skin, respectively, we show that beam self-reconstruction is indeed possible. We demonstrate that a Bessel beam is unexpectedly robust against deflection at objects, and we define measures for self-reconstruction in this context. We present a prototype of a microscope with self-reconstructing beams (MISERB) and show that a holographically shaped, scanned Bessel beam not only reduces scattering artefacts, but also simultaneously increases image quality and penetration depth in dense media.