Culture conditions of Roseobacter strain 27-4 affect its attachment and biofilm formation as quantified by real-time PCR
Culture conditions of Roseobacter strain 27-4 affect its attachment and biofilm formation as quantified by real-time PCR
复制标题
DOI：
10.1128/aem.72.4.3011-3015.2006
复制
发表时间：
2006-04-01
影响因子：
4.4
通讯作者：
Gram, L
中科院分区：
文献类型：
--
作者：
Bruhn, JB;Haagensen, JAJ;Gram, L
鱼益生菌Roseobacterium菌株27-4仅以轮虫形式生长，并在静态生长条件下产生其抗菌化合物。它在静态条件下预培养时形成三维生物膜。我们使用直接实时PCR方法定量Rosefilm菌株27-4的附着，并证明细菌在静态生长期间比在通气条件下更有效地附着于表面。
The fish probiotic bacterium Roseobacter strain 27-4 grows only as rosettes and produces its antibacterial compound under static growth conditions. It forms three-dimensional biofilms when precultured under static conditions. We quantified attachment of Roseobacter strain 27-4 using a direct real-time PCR method and demonstrated that the bacteria attached more efficiently to surfaces during static growth than under aerated conditions.