Versican expression is associated with chamber specification, septation, and valvulogenesis in the developing mouse heart
Versican expression is associated with chamber specification, septation, and valvulogenesis in the developing mouse heart
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DOI：
10.1161/01.res.83.5.523
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发表时间：
1998-09-07
影响因子：
20.1
通讯作者：
Copp, AJ
中科院分区：
文献类型：
--
作者：
Henderson, DJ;Copp, AJ
versican （PG-M）基因编码一种硫酸软骨素蛋白聚糖，这种蛋白聚糖不允许细胞迁移，并且与细胞增殖和细胞分化缓慢有关。利用原位杂交和免疫细胞化学技术对小鼠胚胎切片进行分析，我们发现versican的mRNA和蛋白在心脏发育过程中具有相似的分布，并以动态模式表达。Versican在管状心脏中广泛表达，但在心房中迅速下调，在室间隔发生前，右心室的转录水平高于左心室。Versican在小梁状心室心肌强烈表达，而致密增生区转录本丰度较低。它在室间隔的外层和嵴上表达，在主房间隔的间质帽上也很突出。Versican在房室和流出道区域的心内膜缓冲层以及房室、半月瓣和静脉瓣膜中表达尤其强烈。本研究提出了Versican可能参与室室的形成、房间隔和室间隔的生长和融合，以及心内膜缓冲层发育特征从上皮细胞向间质细胞的转化的可能性。Versican可能是心脏发生的关键参与者，对许多介导发育中的心内膜和心肌之间相互作用的扩散信号作出反应。
The versican (PG-M) gene encodes a chondroitin sulfate proteoglycan that is nonpermissive for cell migration and appears in association with slow cell proliferation and cytodifferentiation. Using the techniques of in situ hybridization and immunocytochemistry on sectioned mouse embryos, we found that the mRNA and protein for versican show similar distributions and are expressed in a dynamic pattern during development of the heart. Versican exhibits generalized expression in the tubular heart but becomes rapidly downregulated in the atrium and exhibits higher transcript levels on the right side of the ventricular chamber than the left, before the onset of ventricular septation. Versican is expressed strongly in the trabeculated ventricular myocardium, whereas the compact proliferative zone has lower transcript abundance. It is expressed in the outer layers and on the crest of the ventricular septum and is prominent on the mesenchymal cap of the primary atrial septum. Versican is particularly strongly expressed in the endocardial cushions of the atrioventricular and outflow tract regions and in the atrioventricular, semilunar, and venous valves, This study raises the possibility that versican may be involved in specification of the ventricular chambers, in growth and fusion of the atrial and ventricular septa, and in the transformation from epithelium to mesenchyme that characterizes development of the endocardial cushions. Versican may be a key participant in cardiogenesis, responding to the many diffusible signals that mediate interactions between the developing endocardium and myocardium.