Immunoisolated chromaffin cells implanted into the subarachnoid space of rats reduce cold allodynia in a model of neuropathic pain: A novel application of microencapsulation technology
Immunoisolated chromaffin cells implanted into the subarachnoid space of rats reduce cold allodynia in a model of neuropathic pain: A novel application of microencapsulation technology
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DOI：
10.1111/j.1525-1594.2004.00024.x
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发表时间：
2004-12-01
影响因子：
2.4
通讯作者：
Baek, WY
中科院分区：
文献类型：
--
作者：
Kim, YM;Jeon, YH;Baek, WY
肾上腺髓质嗜铬细胞的鞘内移植通过分泌儿茶酚胺、阿片类物质和其他神经活性物质来缓解慢性疼痛。近年来，人们利用半透性膜大胶囊分离免疫异种嗜铬细胞，但这种方法的有效性受到限制，在体内的封装嗜铬细胞的生存能力差。在这项研究中，我们使用了一种新的封装方法来增加嗜铬细胞的活力。我们发现，微囊化嗜铬细胞植入大鼠蛛网膜下腔缓解了神经病理性疼痛模型的冷异常性疼痛。此外，微囊化嗜铬细胞形态正常，并保留其功能。这些发现表明，鞘内放置微囊化嗜铬细胞可能是一种有用的方法，用于治疗慢性疼痛。
Intrathecal transplants of adrenal medullary chromaffin cells relieve chronic pain by secreting catecholamines, opioids, and other neuroactive substances. Recently, macrocapsules with semipermeable membranes were used to isolate immunologically xenogenic chromaffin cells, but the poor viability in vivo of the encapsulated chromaffin cells limited the usefulness of this method. In this study, we used a novel method of encapsulation to increase the viability of chromaffin cells. We found that microencapsulated chromaffin cells that were implanted into the subarachnoid space of rats relieved cold allodynia in a model of neuropathic pain. Furthermore, microencapsulated chromaffin cells were morphologically normal and retained their functionality. These findings suggest that the intrathecal placement of microencapsulated chromaffin cells might be a useful method for treating chronic pain.