Effects of Angelica sinensis rhizosphere soil on A.sinensis seed germination and seedling growth
Effects of Angelica sinensis rhizosphere soil on A.sinensis seed germination and seedling growth
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作者：
Li Yi-ying
为探讨当归连作障碍的自毒作用，研究了当归水提液的化感作用。中华猕猴桃根际土壤采用化感效应指数（RI）、综合化感效应指数（M）和方差分析等方法，对不同品种的化感作用进行了研究。结果表明，A.根际和非根际土壤对黄芪种子萌发、幼苗生长和酶活性均有明显的抑制作用。与蒸馏水和未种植的土壤水提液比较。A.根际土壤浸提液随质量浓度的增加而增加，不同质量浓度（0.125-1.000 g·ml-1）之间差异显著（P0.05）。在相同质量浓度下，根际土壤浸提液的作用明显大于非根际土壤浸提液。质量浓度为0.500 g·ml-1的根际和非根际土壤浸提液对猕猴桃的SOD、POD、CAT和NR活性均有抑制作用。两种土壤浸提液的抑制作用差异显著（P0. 05）。研究了根际土壤、非根际土壤和未种植土壤水浸液对黄芪种子萌发、幼苗生长和酶活性的抑制作用。结果表明，自毒作用可能是导致猕猴桃连作障碍的原因之一。中国的。
To investigate the autotoxicity of continuous cropping obstacle of Angelica sinensis, the allelopathic effects of the aqueous extract of A. sinensis rhizosphere soil on A. sinensis seed germination, seedling growth, and physiological indices were determined by applying allelopathic effect index (RI), integrated allelopathic effect index (M), and variance analysis. It was shown that the aqueous extracts of A. sinensis rhizosphere and nonrhizosphere soils had significant inhibitory effects on the seed germination, seedling growth, and enzyme activities of A. sinensis, compared with distilled water and unplanted soil aqueous extract. The integrated allelopathic effects of A. sinensis rhizosphere soil extract increased with its increasing mass concentration, and had significant differences (P0.05) between various mass concentrations (0.125-1.000 g·ml-1). At the same mass concentrations, the effects of rhizosphere soil extract were more distinct than those of nonrhizosphere soil extract. The rhizosphere and nonrhizosphere soil extracts at the mass concentration of 0.500 g·ml-1 inhibited the SOD, POD, CAT and NR activities of A. sinensis seedlings significantly, and there was a significant difference (P0.05) in the inhibitory effects between the two soil extracts. The inhibitory effect of the aqueous extracts of rhizosphere-, nonrhizosphere- and unplanted soils on the seed germination, seedling growth, and enzymatic activities of A. sinensis was in the order of rhizosphere soilnonrhizosphere soilunplanted soil, indicating that autotoxicity could be one of the reasons of continuous cropping obstacle of A. sinensis.