A peptide hormone and its receptor protein kinase regulate plant cell expansion.
A peptide hormone and its receptor protein kinase regulate plant cell expansion.
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DOI：
10.1126/science.1244454
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发表时间：
2014-01-24
期刊：
影响因子：
--
通讯作者：
Sussman MR
中科院分区：
文献类型：
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作者：
Haruta M;Sabat G;Stecker K;Minkoff BB;Sussman MR
植物细胞是不动的，因此，植物的生长和发育取决于细胞的扩张，而不是细胞的迁移。质膜引发细胞扩张速率变化的分子机制仍然难以捉摸。我们发现，分泌肽，RALF（快速碱化因子），抑制细胞伸长的主根通过激活细胞表面受体FERONIA在拟南芥。RALF与FERONIA的特异性结合以及FERONIA突变体对RALF诱导的生长抑制的结合减少和不敏感性支持直接肽-受体相互作用。磷酸化蛋白质组测量表明RALF-FERONIA相互作用导致质膜H+-腺苷三磷酸酶2在Ser 899处磷酸化，介导质子转运的抑制。结果揭示了RALF诱导的细胞外碱化的分子机制和调节细胞扩增的信号通路。
Plant cells are immobile; thus, plant growth and development depend on cell expansion rather than cell migration. The molecular mechanism by which the plasma membrane initiates changes in the cell expansion rate remains elusive. We found that a secreted peptide, RALF (rapid alkalinization factor), suppresses cell elongation of the primary root by activating the cell surface receptor FERONIA in Arabidopsis thaliana. A direct peptide-receptor interaction is supported by specific binding of RALF to FERONIA and reduced binding and insensitivity to RALF-induced growth inhibition in feronia mutants. Phosphoproteome measurements demonstrate that the RALF-FERONIA interaction causes phosphorylation of plasma membrane H+–adenosine triphosphatase 2 at Ser899, mediating the inhibition of proton transport. The results reveal a molecular mechanism for RALF-induced extracellular alkalinization and a signaling pathway that regulates cell expansion.