The effect of different concentrations of topical ozone administration on bone formation in orthopedically expanded suture in rats
The effect of different concentrations of topical ozone administration on bone formation in orthopedically expanded suture in rats
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DOI：
10.1093/ejo/cjv045
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发表时间：
2016-06-01
影响因子：
2.6
通讯作者：
Sonmez, Mehmet Fatih
中科院分区：
文献类型：
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作者：
Buyuk, S. Kutalmis;Ramoglu, Sabri Ilhan;Sonmez, Mehmet Fatih
本研究的目的是探讨不同浓度的臭氧 (O-3) 治疗对大鼠前颌间缝扩张后骨再生的影响。 将 48 只 Wistar 大鼠随机分为 4 组 (n = 12)。在I、II和III组中，分别在前颌缝处注射1ml 10、25和40μg/ml的O-3。 IV组（对照组），在扩张过程中的同一点注射1ml盐水溶液，持续5天。通过组织形态计量学评估缝合线中的骨再生。测量并比较新生骨和纤维化面积、成骨细胞和破骨细胞的数量以及血管数量。通过使用锥形束计算机断层扫描来测量扩张区域中新形成的骨的密度。数据采用Kruskal-Wallis单因素方差分析和事后Student-Newman-Keuls检验进行分析。实验组的新骨面积、纤维化面积、成骨细胞和破骨细胞数量以及血管数量均显着高于对照组（P < 0.001）。与其他实验组和对照组相比，25μg/ml O-3组上颌骨缝中新生骨密度（P < 0.001）、新骨形成（P = 0.009）、毛细血管数量（P < 0.001）、破骨细胞数量（P = 0.016）和成骨细胞数量（P < 0.001）最高。 O-3 疗法可以在扩张和保留期间刺激骨科扩张的前颌间缝合线中的骨再生。
The aim of this study was to investigate the effects of different concentrations of ozone (O-3) therapy on bone regeneration in response to an expansion of the inter-premaxillary suture in rats.Forty-eight Wistar rats were randomly divided into four groups (n = 12). In groups I, II, and III, 1ml of O-3 at 10, 25, and 40 A mu g/ml was injected at the premaxillary suture, respectively. In group IV (control group), 1ml of saline solution was injected at the same point during the expansion procedure for 5 days. Bone regeneration in the suture was evaluated histomorphometrically. The area of new bone and fibrotic area, the number of osteoblasts and osteoclasts, and the amount of vascularity were measured and compared. The density of the newly formed bone in the expansion area was measured by using cone beam computed tomography. Data were analyzed using the Kruskal-Wallis one-way analysis of variance and post hoc Student-Newman-Keuls tests.New bone area, fibrotic area, osteoblast and osteoclast numbers, and the amount of vascularity were significantly higher in experimental groups compared with the control group (P < 0.001). The density of newly formed bone (P < 0.001), new bone formation (P = 0.009), number of capillaries (P < 0.001), number of osteoclasts (P = 0.016), and number of osteoblasts (P < 0.001) in the maxillary sutures were highest in the 25 mu g/ml O-3 group compared with the other experimental groups and control group.The application of O-3 therapy can stimulate bone regeneration in an orthopedically expanded inter-premaxillary suture during both the expansion and retention periods.