Helicobacter felis and Helicobacter bizzozeronii induce gastric parietal cell loss in Mongolian gerbils
Helicobacter felis and Helicobacter bizzozeronii induce gastric parietal cell loss in Mongolian gerbils
复制标题
DOI：
10.1016/j.micinf.2005.08.003
复制
发表时间：
2006-02-01
影响因子：
5.8
通讯作者：
Ducatelle, R
中科院分区：
文献类型：
--
作者：
De Bock, M;Decostere, A;Ducatelle, R
人类非幽门螺杆菌感染与胃炎、胃溃疡和MALT淋巴瘤有关，其中约50%是由猫狗中常见的螺杆菌引起的，包括猫螺杆菌、毕氏螺杆菌和幽门螺杆菌。salomonis。与幽门螺杆菌不同，这些菌的毒力机制尚不清楚。felis、猫H. bizzozeronii和H. salomonis在蒙古沙鼠中进行了研究。雌性SPF沙鼠灌胃接种H. felis、猫H. bizzozeronii或H. salomonis并在3周后处死。取胃底和胃窦标本进行PCR细菌学检测。取覆盖所有胃区域的纵向条带进行组织学检查。评估胃定植、炎症、细胞凋亡、壁细胞损失和细胞增殖。对照组和H.接种沙门氏菌的沙鼠PCR均为阴性。H. felis和H. bizzozeronii接种动物均为阳性，H.猫接种的动物显示从限制嵴延伸到胃底的壁细胞的损失。在没有壁细胞的粘膜区域观察到高细胞增殖率。在受影响的壁细胞和正常壁细胞之间的过渡区可见凋亡细胞和大量螺杆菌的密集带。In H. bizzozeronii感染沙鼠，这是不太明显。胃上皮细胞的局灶性凋亡丢失与细菌的存在在空间上相关，尤其是在H。felis和H. bizzozeronii感染了沙鼠。这种细胞的损失可能导致肠化生。(c)2005年，Elsevier SAS。All rights reserved.
Non-pylori helicobacter infections are associated with gastritis, gastric ulcers and MALT lymphomas in man. Approximately 50% of these are caused by helicobacters commonly found in dogs and cats, including Helicobacter felis, Helicobacter bizzozeronii and H. salomonis. In contrast to Helicobacter pylori, the virulence mechanisms of these species are unknown, In this study the virulence of H. felis, H. bizzozeronii and H. salomonis was investigated in Mongolian gerbils. Female SPF gerbils were inoculated intragastrically with H. felis, H. bizzozeronii or H. salomonis and sacrificed 3 weeks later. Fundus and antrum samples were taken for bacterial detection by PCR. A longitudinal strip covering all stomach regions was taken for histology. Gastric colonization, inflammation, apoptosis, loss of parietal cells and cell proliferation were assessed. Controls and H. salomonis inoculated gerbils were negative in PCR. H. felis and H. bizzozeronii inoculated animals were positive, H. felis inoculated animals showed loss of parietal cells extending from the limiting ridge into the fundus. A high cell proliferation rate was noticed in the mucosal area devoid of parietal cells. A dense band of apoptotic cells and large numbers of Helicobacter bacteria were seen at the transition zone between affected and normal parietal cells. In H. bizzozeronii infected gerbils, this was less pronounced. Focal apoptotic loss of gastric epithelial cells was spatially associated with the presence of bacteria especially in H. felis and to a lesser extent in H. bizzozeronii infected gerbils. This loss of cells may lead to intestinal metaplasia. (c) 2005 Elsevier SAS. All rights reserved.