The electron beam deposition of titanium on polyetheretherketone (PEEK) and the resulting enhanced biological properties
The electron beam deposition of titanium on polyetheretherketone (PEEK) and the resulting enhanced biological properties
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DOI：
10.1016/j.biomaterials.2009.12.030
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发表时间：
2010-05-01
期刊：
影响因子：
14
通讯作者：
Kuh, Sung-Uk
中科院分区：
文献类型：
--
作者：
Han, Cheol-Min;Lee, Eun-Jung;Kuh, Sung-Uk
采用电子束沉积法在聚醚醚酮（PEEK）表面涂覆纯钛（Ti）层，以增强其生物相容性和与骨组织的粘附性。电子束沉积法是一种低温涂层工艺，可形成致密、均匀且结晶良好的Ti层，而不会劣化PEEK植入物的特性。Ti涂层与基体结合牢固，润湿性显著提高。钛涂层样品的体外细胞行为和体内骨整合方面进行了评价，并将结果与纯PEEK基材进行了比较。使用甲氧苯基四唑鎓盐（MTS）测定法测量细胞（MC 3 T3-E1）的增殖水平，并且在Ti涂层后增殖水平增加一倍以上。使用碱性磷酸酶（ALP）测定法测量细胞的分化水平，并且也加倍。此外，体内动物试验表明，钛涂层PEEK植入物的骨接触（BIC）比比纯PEEK植入物高得多。这些体外和体内结果表明，电子束沉积的钛涂层显著提高了PEEK用于硬组织应用的潜力。(C)2009爱思唯尔有限公司保留所有权利。
The surface of polyetheretherketone (PEEK) was coated with a pure titanium (Ti) layer using an electron beam (e-beam) deposition method in order to enhance its biocompatibility and adhesion to bone tissue. The e-beam deposition method was a low-temperature coating process that formed a dense, uniform and well crystallized Ti layer without deteriorating the characteristics of the PEEK implant. The Ti coating layer strongly adhered to the substrate and remarkably enhanced its wettability. The Ti-coated samples were evaluated in terms of their in vitro cellular behaviors and in vivo osteointegration, and the results were compared to a pure PEEK substrate. The level of proliferation of the cells (MC3T3-E1) was measured using a methoxyphenyl tetrazolium salt (MTS) assay and more than doubled after the Ti coating. The differentiation level of cells was measured using the alkaline phosphatase (ALP) assay and also doubled. Furthermore, the in vivo animal tests showed that the Ti-coated PEEK implants had a much higher bone-in-contact (BIC) ratio than the pure PEEK implants. These in vitro and in vivo results suggested that the e-beam deposited Ti coating significantly improved the potential of PEEK for hard tissue applications. (C) 2009 Elsevier Ltd. All rights reserved.