Estimation of refractive index of eggshell in intact egg using terahertz time-domain spectroscopy
Estimation of refractive index of eggshell in intact egg using terahertz time-domain spectroscopy
复制标题
使用太赫兹时域光谱估计完整鸡蛋中蛋壳的折射率
DOI：
10.1117/12.2585199
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Y
中科院分区：
文献类型：
--
作者：
Khaliduzzaman;A.;Shiraga;K.;Kondo;N.;and Ogawa;Y
太赫兹是一种相对较新的东西，它如何与许多生物物体相互作用，如鸟类蛋壳，还有待探索。蛋壳保护鸡蛋的内部内容物免受污染和紫外线辐射。它也是胚胎发育的主要矿物质来源。表明蛋壳致密性的折射率可以作为蛋壳的潜在质量指标，无论是对肉鸡还是对孵化的鸡蛋。但人们对蛋壳的折射率知之甚少。然而，现有的信息只是基于破坏性的方法，在许多情况下不适合使用，因为丢失了蛋壳的完整性。因此，我们的目标是开发一种利用太赫兹时间域光谱(THz-TDS)无损测量蛋壳折射率的方案，它可以直接从时间脉冲中获得太赫兹区域的折射率。为了消除蛋清对太赫兹的强烈吸收，我们用THz-TDS测量了完整蛋壳在0.2-1.2 THz范围内的反射率。THz波的频域干扰信号包含了蛋壳厚度和折射率的信息。结果表明，该方法可用于完整蛋壳折射率的估算，决定系数R2为0.49。该方法不仅可用于鸡蛋的工业分级，还可用于应用光学、鸟类学、进化生物学等多个应用研究领域。
Terahertz is a relatively new, and yet to be explored that how it interacts with many biological objects like avian eggshell. Eggshell protects internal contents of egg from contaminants and ultraviolet radiation. It also acts as a major mineral source for embryonic development. The refractive index indicating eggshell compactness could be a potential quality criterion of shell for both table and hatching egg. But a little is known about refractive index of eggshell. However, the available information is only based on destructive method which is not suitable to use in many cases due to loss of eggshell integrity. Therefore, we aim to develop a non-destructive protocol of eggshell refractive index measurement using terahertz timedomain spectroscopy (THz-TDS) which can directly derive refractive index in the terahertz region from the temporal pulses. In order to get rid of intense terahertz absorption by egg albumen, we used THz-TDS to measure reflectance of intact eggshell in the 0.2-1.2 THz. THz wave`s frequency domain interferences signal contains the information of both thickness and refractive index of the eggshell. The results revealed that this proposed method can be used in estimation of refractive index of intact eggshell with co-efficient of determination (R2) of 0.49. This non-destructive protocol could not only be used in industrial grading of eggs but also in many applied research fields such as applied optics, ornithology, evolutionary biology in the future.