Phylogenetics and phylogeography of the monocot genus Baldellia (Alismataceae): Mediterranean refugia, suture zones and implications for conservation
Phylogenetics and phylogeography of the monocot genus Baldellia (Alismataceae): Mediterranean refugia, suture zones and implications for conservation
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DOI：
10.1016/j.ympev.2010.11.009
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发表时间：
2011-01-01
影响因子：
4.1
通讯作者：
Kozlowski, Gregor
中科院分区：
文献类型：
--
作者：
Arrigo, Nils;Buerki, Sven;Kozlowski, Gregor
水生植物，特别是具有象征意义的巴尔代利亚属（泽泻科），是最受威胁的生物之一，由于前所未有的人类驱动的栖息地破坏。因此，保护计划是至关重要的，并呼吁彻底记录遗传多样性和澄清这一濒危属的分类。我们的样本包括来自42个自然种群的282个个体，覆盖了整个欧洲和地中海的属的地理范围。为了研究泽泻科植物的亲缘关系，结合核内转录间隔区（ITS）和叶绿体trnL-ndhF区的序列分析和AFLP基因分型，建立了泽泻属植物的地理分布图。我们的亲缘关系强烈支持Baldellia的单系性，并将其作为Luronium和Alisma的姐妹分支，因此排除了，如先前所假设的，与主要新热带属Echinodorus的密切遗传关系。巴尔代利亚的冰川地理学勾勒出的模式与一个假设相一致，该假设认为位于伊比利亚半岛和意大利/巴尔干半岛的地区的冰川避难所是两个不同的遗传谱系重新殖民欧洲的地方。这两个谱系分别对应于Baldellia ranunculoides（意大利/巴尔干半岛的衍生群体）和Baldellia repens（从伊比利亚半岛避难所恢复的群体），因此支持在以前的生态和形态学研究中概述的两个类群之间的差异。这些结果通过确认B的遗传特异性澄清了分类学上的不确定性。根据B进行匍匐。毛茛状。第三个谱系Baldellia alpestris起源于伊比利亚半岛的山区，至今仍是该地区的特有种。出乎意料的是，B。虽然在北方非洲采集到的100个匍匐茎种群在遗传上与欧洲的同类种群不同，但这需要进一步的调查，以充分解决它们的遗传和保护状况。最后，我们在欧洲西北部发现了一个大的B之间的杂交区。repens和B.毛茛状。这些结果进行了讨论，根据Baldellia种群的保护措施。(C)2010年爱思唯尔公司All rights reserved.
Aquatic plants, and especially the emblematic genus Baldellia (Alismataceae), are among the most threatened organisms, due to unprecedented human-driven habitat destructions. Therefore protection plans are crucially needed and call for thoroughly documenting the genetic diversity and clarifying the taxonomy of this endangered genus. Our sampling included 282 individuals from 42 natural populations and covered the whole geographical range of the genus, across Europe and the Mediterranean. We combined sequencing of nuclear internal transcribed spacer (ITS) and chloroplastic trnL-ndhF regions with amplified fragment length polymorphism (AFLP) genotyping to investigate the Alismataceae phylogeny, and produce a phylogeography of Baldellia. Our phylogeny strongly supported the monophyly of Baldellia and placed it as the sister clade to Luronium and Alisma, therefore excluding, as previously supposed, a close genetic relatedness to the predominantly neotropical genus Echinodorus. The phylogeography of Baldellia outlined patterns consistent with a hypothesis considering glacial refugia located in the Iberian Peninsula and the Italy/Balkan region from which two distinct genetic lineages re-colonized Europe. These two lineages corresponded respectively to Baldellia ranunculoides (Italy/Balkan derived populations) and Baldellia repens (populations recovered from the Iberian Peninsula refuge), therefore supporting differences outlined between the two taxa in previous ecological and morphological studies. These results allowed clarifying taxonomic uncertainties by confirming the genetic distinctness of B. repens according to B. ranunculoides. A third lineage, Baldellia alpestris, originated and remained endemic to the mountainous regions of the Iberian Peninsula. Unexpectedly, B. repens populations collected in northern Africa, appeared to be genetically distinct from their European counterparts, this calls for further investigation to fully address their genetic and conservation status. Finally, we detected a large hybridization zone in northwestern Europe between B. repens and B. ranunculoides. These results were discussed in light of conservation approaches for Baldellia populations. (C) 2010 Elsevier Inc. All rights reserved.