Phosphatidate phosphohydrolase in purified rat brain myelin.
Phosphatidate phosphohydrolase in purified rat brain myelin.
复制标题
纯化的大鼠脑髓磷脂中的磷脂酸磷酸水解酶。
DOI：
10.1002/jnr.490240313
复制
发表时间：
1989
影响因子：
4.2
通讯作者：
Ledeen,RW
中科院分区：
文献类型：
--
作者：
Vaswani,KK;Ledeen,RW
高纯度的大鼠脑干髓鞘被证明含有磷脂酸盐磷酸水解酶，这是一种将磷脂酸盐转化为二酰甘油的酶。相对于细胞质和微生物体的高水平(分别为17%和22%)，倾向于排除这些组分作为活性来源的污染。另外的证据来自对反复提纯、混合实验以及用盐和洗涤剂洗涤髓鞘的研究。我们得出的结论是，这种酶除了广泛分布在其他亚细胞组分中外，还是髓鞘膜的固有酶。通过它的活性，它产生了胞苷(肯尼迪)途径的一个关键底物，这是以前被证明发生在该膜上的。
Highly purified myelin from rat brain stem has been shown to contain phosphatidate phosphohydrolase, an enzyme which converts phosphatidate to diacylglycerol. The high levels relative to cytosol and microsomes (17% and 22%, respectively) tended to preclude contamination by these fractions as the source of activity. Additional evidence came from study of repeated purification, mixing experiments, and washing of the myelin with salt and detergent. We conclude that this enzyme, in addition to being widely distributed in other subcellular fractions, is intrinsic to the myelin membrane. Through its activity it generates a key substrate for the cytidine (Kennedy) pathway which was previously shown to occur in this membrane.