Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.
Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.
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小鼠胚胎细胞在培养物及其发展成既定线的生长的定量研究。
DOI：
10.1083/jcb.17.2.299
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发表时间：
1963-05
影响因子：
7.8
通讯作者：
GREEN, H
中科院分区：
文献类型：
--
作者：
TODARO, G J;GREEN, H
在单层中生长的解聚的小鼠胚胎细胞在连续转移后生长速率逐渐下降，下降的速度除其他外取决于接种密度。然而，几乎所有的文化发展成为建立线内3个月的文化。建立的细胞系出现的第一个标志是细胞保持恒定或上升的潜在生长速率的能力。这发生在培养物形态学未改变时。生长速率继续增加，直到其等于或超过原始培养物的生长速率。早期建立的细胞表现出越来越多的代谢自主性，如通过减少对细胞间喂养的依赖所示。有人建议，建立的过程涉及细胞渗透性的改变。染色体研究表明，负责增长率上升的细胞是二倍体，但后来人口转移到四倍体范围，往往非常迅速。再后来，标记染色体出现了。不同的线获得不同的属性，这取决于所采用的培养条件;一个线开发，这是非常敏感的接触抑制。
Disaggregated mouse embryo cells, grown in monolayers, underwent a progressive decline in growth rate upon successive transfer, the rapidity of the decline depending, among other things, on the inoculation density. Nevertheless, nearly all cultures developed into established lines within 3 months of culture. The first sign of the emergence of an established line was the ability of the cells to maintain a constant or rising potential growth rate. This occurred while the cultures were morphologically unchanged. The growth rate continued to increase until it equaled or exceeded that of the original culture. The early established cells showed an increasing metabolic autonomy, as indicated by decreasing dependence on cell-to-cell feeding. It is suggested that the process of establishment involves an alteration in cell permeability properties. Chromosome studies indicated that the cells responsible for the upturn in growth rate were diploid, but later the population shifted to the tetraploid range, often very rapidly. Still later, marker chromosomes appeared. Different lines acquired different properties, depending on the culture conditions employed; one line developed which is extremely sensitive to contact inhibition.