Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy
Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy
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DOI：
10.1063/1.2148996
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发表时间：
2006-01-01
影响因子：
1.6
通讯作者：
Girkin, JM
中科院分区：
文献类型：
--
作者：
McConnell, G;Poland, S;Girkin, JM
我们报道了使用计算机控制的数字微镜装置（DMD）从白光超连续体中选择离散波长范围，并将该光源应用于共聚焦激光扫描显微镜（CLSM）。DMD的快速切换速率使视频速率波长调谐成为可能，而高分辨率允许对带宽选择进行精确控制。为了证明该系统的有效性和实际应用，在对各种多标记样品进行三维CLSM之前，将DMD系统与CLSM系统结合使用来表征波长切换能力。(c) 2006年美国物理研究所。
We report on the use of a computer-controlled digital micromirror device (DMD) to select discrete wavelength ranges from a white-light supercontinuum and the application of the light source to confocal laser scanning microscopy (CLSM). The fast switching rate of the DMD enabled video-rate wavelength tuning, while the high resolution allowed precise control over the bandwidth selection. The DMD system was used in conjunction with a CLSM system to characterize the wavelength switching capability, prior to performing three-dimensional CLSM of a variety of multiple-labeled samples in order to demonstrate the efficacy and practical application of the system. (c) 2006 American Institute of Physics.