GENETIC DIVERSITY IN POPULATIONS OF CISTOPUS SP. BASED ON THE SEQUENCES OF MITOCHONDRIAL 16S rRNA AND COI GENE FRAGMENTS
GENETIC DIVERSITY IN POPULATIONS OF CISTOPUS SP. BASED ON THE SEQUENCES OF MITOCHONDRIAL 16S rRNA AND COI GENE FRAGMENTS
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发表时间：
2009
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通讯作者：
Yang Qiu
中科院分区：
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作者：
Yang Qiu
对中国东海5个种群的16S rRNA和COⅠ基因片段进行了序列分析，获得了16S rRNA基因497~501bp和COⅠ基因652个碱基的核苷酸序列，其中A+T碱基组成比例较高。同源性分析表明，种群内变异水平很低。在5个种群中共检测到8个变异位点(其中7个为插入序列)，在5个种群中检测到21个Ⅰ基因片段的多态位点。在5个种群中检测到8个变异位点，其中7个为插入序列NJ和UPGMA的系统发育树表明，这些种群之间没有明显的遗传差异。
Sequences of 16S rRNA and COⅠ gene fragments in mtDNA of Cistopus sp.were studied.The samples were collected from five populations in the East China Sea.The sequences were compared by PCR.Nucleotide sequences of 497—501bp for 16S rRNA gene and 652bp for COⅠ gene were obtained.A high percentage of A+T base composition in the two sequences was observed.The alignment indicated that the variation level of intra-stocks was very low in both genes.Eight variable sites were detected for 16S rRNA gene fragment(of which seven were indels) across the five stocks.21 polymorphic sites were detected in COⅠ gene fragments among the five stocks,with no indels.The NJ and UPGMA phylogenetic trees showed that no remarkable genetic difference was observed among those populations.