Combinatorial Regulation of Stilbene Synthase Genes by WRKY and MYB Transcription Factors in Grapevine (Vitis vinifera L.)
Combinatorial Regulation of Stilbene Synthase Genes by WRKY and MYB Transcription Factors in Grapevine (Vitis vinifera L.)
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DOI：
10.1093/pcp/pcy045
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发表时间：
2018-05
影响因子：
4.9
通讯作者：
A. Vannozzi;D. Wong;Janine H[REPLACEMENT CHARACTER]ll;Ibrahim Hmmam;J. T. Matus;J. Bogs;Tobias Ziegler;I. Dry;G. Barcaccia;M. Lucchin
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文献类型：
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作者：
A. Vannozzi;D. Wong;Janine H[REPLACEMENT CHARACTER]ll;Ibrahim Hmmam;J. T. Matus;J. Bogs;Tobias Ziegler;I. Dry;G. Barcaccia;M. Lucchin
芪合酶（STS）是白藜芦醇生物合成的关键酶。最近我们报道了两个调控葡萄藤二苯乙烯生物合成途径的R2 R3-MYB转录因子（TF）基因：VviMYB 14和VviMYB 15。这些基因在一系列的压力和发育条件下与STS基因强烈共表达，这与这些TF对STS启动子的特异性激活一致。基于微阵列和RNA测序数据，使用两个单独的转录组纲要进行全基因组基因共表达分析，结果显示WRKY TF是与STS基因相关的顶级TF家族。在相关频率的基础上，进一步筛选出4个WRKY基因，即VviWRKY 03、VviWRKY 24、VviWRKY 43和VviWRKY 53，并进行功能验证。在非应激和应激条件下的表达分析，连同启动子-荧光素酶报告基因测定，表明这些TF在芪生物合成途径的调节中的不同层次。特别地，VviWRKY 24似乎在VviSTS 29启动子的激活中充当单一效应子，而VviWRKY 03通过与VviMYB 14的组合效应起作用，这表明这两种调节剂可能在蛋白质水平上相互作用，如先前在其他物种中报道的那样。
Stilbene synthase (STS) is the key enzyme leading to the biosynthesis of resveratrol. Recently we reported two R2R3-MYB transcription factor (TF) genes that regulate the stilbene biosynthetic pathway in grapevine: VviMYB14 and VviMYB15. These genes are strongly co-expressed with STS genes under a range of stress and developmental conditions, in agreement with the specific activation of STS promoters by these TFs. Genome-wide gene co-expression analysis using two separate transcriptome compendia based on microarray and RNA sequencing data revealed that WRKY TFs were the top TF family correlated with STS genes. On the basis of correlation frequency, four WRKY genes, namely VviWRKY03, VviWRKY24, VviWRKY43 and VviWRKY53, were further shortlisted and functionally validated. Expression analyses under both unstressed and stressed conditions, together with promoter-luciferase reporter assays, suggested different hierarchies for these TFs in the regulation of the stilbene biosynthetic pathway. In particular, VviWRKY24 seems to act as a singular effector in the activation of the VviSTS29 promoter, while VviWRKY03 acts through a combinatorial effect with VviMYB14, suggesting that these two regulators may interact at the protein level as previously reported in other species.