Motor skills training promotes motor functional recovery and induces synaptogenesis in the motor cortex and striatum after intracerebral hemorrhage in rats
Motor skills training promotes motor functional recovery and induces synaptogenesis in the motor cortex and striatum after intracerebral hemorrhage in rats
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DOI：
10.1016/j.bbr.2013.11.034
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发表时间：
2014-03-01
影响因子：
2.7
通讯作者：
Ishida, Kazuto
中科院分区：
文献类型：
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作者：
Tamakoshi, Keigo;Ishida, Akimasa;Ishida, Kazuto
我们研究了运动技能训练对大鼠脑出血（ICH）后几种运动功能和突触可塑性的影响。雄性Wistar大鼠左侧纹状体注射胶原酶诱导ICH，随机分为ICH组和假手术组。每组又分为运动技能训练组（杂技训练）和控制组（无运动）。杂技组于术后4～28天进行杂技训练。在ICH后的几个时间点通过运动缺陷评分、水平梯测试和宽或窄梁行走测试来评估运动功能。使用免疫组织化学计数 Delta FosB 阳性细胞的数量以检查神经元激活，并通过蛋白质印迹分析 PSD95 蛋白水平以检查 ICH 后 14 天和 29 天双侧感觉运动皮层和纹状体的突触可塑性。 ICH后的运动技能训练显着改善了ICH后早期的粗大运动功能和后期的熟练运动协调功能。与对照组相比，杂技组对侧感觉运动皮层中 Delta FosB 阳性细胞的数量显着增加。运动皮层中的PSD95蛋白表达在后期显着增加，而在纹状体中，与ICH后没有训练相比，通过运动技能训练，早期纹状体中的蛋白水平显着增加。我们证明运动技能训练可以改善大鼠脑出血后的运动功能，并增强纹状体和感觉运动皮层的神经活动和突触可塑性。 (C) 2013 Elsevier B.V. 保留所有权利。
We investigated the effects of motor skills training on several types of motor function and synaptic plasticity following intracerebral hemorrhage (ICH) in rats. Male Wistar rats were injected with collagenase into the left striatum to induce ICH, and they were randomly assigned to the ICH or sham groups. Each group was divided into the motor skills training (acrobatic training) and control (no exercise) groups. The acrobatic group performed acrobatic training from 4 to 28 days after surgery. Motor functions were assessed by motor deficit score, the horizontal ladder test and the wide or narrow beam walking test at several time points after ICH. The number of Delta FosB-positive cells was counted using immunohistochemistry to examine neuronal activation, and the PSD95 protein levels were analyzed by Western blotting to examine synaptic plasticity in the bilateral sensorimotor cortices and striata at 14 and 29 days after ICH. Motor skills training following ICH significantly improved gross motor function in the early phase after ICH and skilled motor coordinated function in the late phase. The number of Delta FosB-positive cells in the contralateral sensorimotor cortex in the acrobatic group significantly increased compared to the control group. PSD95 protein expression in the motor cortex significantly increased in the late phase, and in the striatum, the protein level significantly increased in the early phase by motor skills training after ICH compared to no training after ICH. We demonstrated that motor skills training improved motor function after ICH in rats and enhanced the neural activity and synaptic plasticity in the striatum and sensorimotor cortex. (C) 2013 Elsevier B.V. All rights reserved.