Behavioral and biochemical studies of total furocoumarins from seeds of Psoralea corylifolia in the forced swimming test in mice
Behavioral and biochemical studies of total furocoumarins from seeds of Psoralea corylifolia in the forced swimming test in mice
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DOI：
10.1016/j.jep.2004.09.033
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发表时间：
2005-01-15
影响因子：
5.4
通讯作者：
Zhang, L
中科院分区：
文献类型：
--
作者：
Chen, Y;Kong, LD;Zhang, L
以阿米替林和氟西汀为对照，研究了补骨脂呋喃香豆素总甙（TFPC）对小鼠强迫游泳行为学和生化指标的影响。TFPC被发现，以减少不动的持续时间在FST没有伴随的变化，走动，饲养和梳理行为的开放领域的测试。给药3天后，7.5 - 100 mg/kg剂量的TFPC显著缩短了不动时间。较高剂量的疗效超过10和20 mg/kg阿米替林和13 mg/kg氟西汀。治疗7天或14天后，TFPC呈倒U型量效关系，以30 mg/kg剂量作用最大，其作用优于阿米替林和氟西汀。在动物的大脑和肝脏中。TFPC处理14 d后，显著抑制脑单胺氧化酶A和B（MAO-A和MAO-B）活性，并呈倒U型剂量依赖关系，在肝中呈剂量依赖关系。此外，除了最低剂量外，TFPC对MAO-B的作用比MAO-A更强，这与阿米替林和氟西汀相似。此外，TFPC还可以阻止血浆皮质醇水平升高，皮质醇水平是下丘脑-垂体-肾上腺（HPA）轴的一个指标。TFPC显著降低肝脏超氧化物歧化酶（SOD）活性和丙二醛（MDA）水平，这两个指标的氧化应激，提供了一个倒U形的剂量依赖关系。这些结果表明，TEPC具有有效的抗抑郁特性，通过在小鼠FST中的MAO活性、HPA轴作用和氧化应激介导。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
The behavioral and biochemical effects of total furocoumarins from seeds of Psoralea corylifolia (TFPC) were investigated in the forced swimming test (FST) in mice in comparison with amitriptyline and fluoxetine. TFPC was found to reduce the duration of immobility in the FST without accompanying changes in ambulatory, rearing and grooming behaviors in the open-field test. After a 3-day treatment, TFPC at the doses from 7.5 to 100 mg/kg significantly decreased the immobility time. The efficacy of the higher doses exceeded that of amitriptyline at 10 and 20 mg/kg and fluoxetine at 13 mg/kg. After a 7- or 14-day treatment, TFPC exhibited an inverted U-shaped dose-response relations, maximal effects were obtained at 30 mg/kg, when the efficacy appeared to be more than that of amitriptyline and fluoxetine. In animal brain and liver. after 14-day treatment, TFPC significantly inhibited monoamine oxidase A and B (MAO-A and MAO-B) activities with an inverted U-shaped dose-dependent relationship in brain, and with dose-dependent relationship in liver. Moreover, TFPC was more potent for MAO-B than MAO-A, except at the lowest dose, which was similar to amitriptyline and fluoxetine. In addition, TFPC blocked plasma elevated cortisol level, an indicator of the hypothalamic-pituitary-adrenal (HPA) axis. TFPC significantly decreased liver superoxide dismutase (SOD) activity and malondialdehyde (MDA) level, two indicators of oxidative stress, providing an inverted U-shaped dose-dependent relationship. These results suggest that TEPC possesses potent antidepressant properties that are mediated via MAO activity, HPA axis action and oxidative stress in the FST in mice. (C) 2004 Elsevier Ireland Ltd. All rights reserved.