Analytical approaches to investigate transmitter content and release from single secretory vesicles.
Analytical approaches to investigate transmitter content and release from single secretory vesicles.
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研究递质含量和单个分泌囊泡释放的分析方法。
DOI：
10.1007/s00216-010-3698-4
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发表时间：
2010
影响因子：
4.3
通讯作者：
Ewing,AndrewG
中科院分区：
文献类型：
--
作者：
Omiatek,DonnaM;Cans,Ann-Sofie;Heien,MichaelL;Ewing,AndrewG
小泡是神经系统信号传递过程中触发的化学信使的高效储存和释放的主要细胞内单位。这篇综述重点介绍了传统的和新兴的分析方法，这些方法使用显微镜、电化学和光谱学来解析神经递质释放的位置、时间过程和量子含量特征。特别关注突触小泡的研究及其参与细胞通讯的基本分子机制。
The vesicle serves as the primary intracellular unit for the highly efficient storage and release of chemical messengers triggered during signaling processes in the nervous system. This review highlights conventional and emerging analytical methods that have used microscopy, electrochemistry, and spectroscopy to resolve the location, time course, and quantal content characteristics of neurotransmitter release. Particular focus is on the investigation of the synaptic vesicle and its involvement in the fundamental molecular mechanisms of cell communication.