Cultivation of entomopathogenic fungi for the search of antibacterial compounds
Cultivation of entomopathogenic fungi for the search of antibacterial compounds
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DOI：
10.1007/s11046-005-0179-y
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发表时间：
2005-11-01
期刊：
影响因子：
5.5
通讯作者：
Nihira, T
中科院分区：
文献类型：
--
作者：
Lee, SY;Nakajima, I;Nihira, T
昆虫病原真菌是天然生物活性物质的丰富来源。为了建立有利于生物活性物质产生的培养条件，并从昆虫病原真菌中筛选出优良的属作为产生菌，对47种典型昆虫病原真菌进行了产生抗生素活性的能力测试。其中38株(81%)产生抗芽孢杆菌化合物，30株(%)产生抗芽孢杆菌化合物或抗葡萄球菌化合物，表明大多数昆虫病原真菌具有产生抗菌化合物的能力。利用9株代表性菌株(Aschersonia sp.HF724、球孢白僵菌HF338、分枝冬虫夏草HF746、金龟子绿僵菌HF293、黄绿色绿僵菌HF698、里氏拟青霉HF588、烟粉拟青霉HF254、细脚拟青霉HF419和蜡叶黄萎病菌HF238)，对液体培养条件和培养基组分进行了考察。在26℃下，金龟子绿僵菌HF293、黑曲霉HF588和莱卡弧菌HF238对芽孢杆菌和酵母菌有明显的抗菌活性，但只有在昆虫来源的物质存在的情况下才能产生抗菌/抗真菌化合物，这表明昆虫来源的物质的存在触发了昆虫病原真菌抗菌/抗真菌化合物的产生。
Entomopathogenic fungi are a rich source of natural bioactive compounds. To establish cultivation conditions which facilitate the production of bioactive compounds and to select good genera among entomopathogenic fungi as the producer, 47 typical entomopathogenic fungi were tested for their ability to produce antibiotic activity. Thirty-eight strains (81%) and 30 strains (64%) of these fungi produced either anti-Bacillus compounds or anti-Staphylococcus compounds, respectively, indicating that the majority of the entomopathogenic fungi tested possessed the ability to produce antibacterial compounds. Using 9 representative strains (Aschersonia sp. HF724, Beauveria bassiana HF338, Cordyceps ramosopulvinata HF746, Metarhizium anisopliae HF293, Metarhizium flavoviride HF698, Nomuraea rileyi HF588, Paecilomyces fumosoroseus HF254, Paecilomyces tenuipes HF419, and Verticillium lecanii HF238), the cultivation conditions in liquid medium were surveyed with respect to the cultivation procedure and medium composition, particularly in terms of the presence or absence of insect-derived materials. At 26 degrees C, M. anisopliae HF293, N. rileyi HF588, and V. lecanii HF238 strains produced clear antibiotic activity against Bacillus and Saccharomyces, but only in the presence of insect-derived materials, suggesting that the production of antibacterial/antifungal compounds by entomopathogenic fungi is triggered by the presence of insect-derived materials.