A route to de novo domestication of wild allotetraploid rice
A route to de novo domestication of wild allotetraploid rice
复制标题
DOI：
10.1016/j.cell.2021.01.013
复制
发表时间：
2021-03-04
期刊：
影响因子：
64.5
通讯作者：
Li, Jiayang
中科院分区：
文献类型：
--
作者：
Yu, Hong;Lin, Tao;Li, Jiayang
栽培稻品种都是二倍体，水稻多倍体因其在基因组缓冲、活力和环境稳健性方面的优势而一直受到人们的欢迎。然而，一条可行的路线仍然难以捉摸。在这里，我们描述了一种实用的策略，即对野生异源四倍体水稻的从头驯化。通过对异源四倍体野生稻(Oryza Alta，CCDD)的筛选，我们鉴定了一个多倍体水稻1号(PPR1)，并建立了两个重要的资源：(1)高效的组织培养、转化和基因组编辑系统；(2)高质量的基因组组合，每个基因组分为两个亚基因组，分别为12条染色体。利用这些资源，我们表明，通过编辑二倍体水稻中控制这些性状的基因的O.Alta同源，可以快速改良六个重要的农艺性状。我们的结果表明，新驯化的异源四倍体水稻有可能发展成为一种新的主食谷物，以加强世界粮食安全。
Cultivated rice varieties are all diploid, and polyploidization of rice has long been desired because of its advantages in genome buffering, vigorousness, and environmental robustness. However, a workable route remains elusive. Here, we describe a practical strategy, namely de novo domestication of wild allotetraploid rice. By screening allotetraploid wild rice inventory, we identified one genotype of Oryza alta (CCDD), polyploid rice 1 (PPR1), and established two important resources for its de novo domestication: (1) an efficient tissue culture, transformation, and genome editing system and (2) a high-quality genome assembly discriminated into two subgenomes of 12 chromosomes apiece. With these resources, we show that six agronomically important traits could be rapidly improved by editing O. alta homologs of the genes controlling these traits in diploid rice. Our results demonstrate the possibility that de novo domesticated allotetraploid rice can be developed into a new staple cereal to strengthen world food security.