Cytotoxic and antiviral activities of aporphine alkaloids of Magnolia grandiflora L.
Cytotoxic and antiviral activities of aporphine alkaloids of Magnolia grandiflora L.
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DOI：
10.1080/14786410902906959
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发表时间：
2010-01-01
影响因子：
2.2
通讯作者：
Ibrahim, N. A.
中科院分区：
文献类型：
--
作者：
Mohamed, S. M.;Hassan, E. M.;Ibrahim, N. A.
从木兰科植物广玉兰叶的甲醇提取物中分离得到四种阿朴吗啡生物碱，分别为木兰花碱、羊毛肌苷、利洛地宁和番荔枝碱。用Hela(宫颈癌细胞系)、HepG2(肝细胞癌细胞系)和U251(脑瘤细胞系)细胞活力测定了木兰花碱和羊毛肌苷的细胞毒作用。与标准多柔比星(IC50)(IC50为0.27)相比，厚朴花碱对HepG2的细胞毒作用(IC50为0.4mgmL-1)强于羊毛苷(IC502.5mgmL-1)。此外，厚朴花碱和羊毛苷对U251细胞也有一定的细胞毒作用，IC50分别为7和4mU g·m L-1。这两个化合物被发现对Hela癌细胞没有活性。另一方面，甲醇提取物对单纯疱疹病毒(HSV-1)表现出较高的抗病毒活性，在1.1mgmL-1时抑制率为76.7%，而对脊髓灰质炎病毒1型的抑制率为中等(相同浓度下抑制率为47%)。这项化学和生物调查以前从未被研究过。
Bioassay guided fractionation of the methanol extract of Magnolia grandiflora L. (Magnoliaceae) leaves led to the isolation and characterisation of four aporphine alkaloids, magnoflorine, lanuginosine, liriodenine and anonaine. The cytotoxicities of the pure compounds magnoflorine and lanuginosine were determined in a cell viability assay with the tumour cell lines Hela (cervix tumour cell line), HEPG2 (hepatocellular carcinoma cell line) and U251 (brain tumour cell line). Magnoflorine was more cytotoxic (IC50 0.4 mu g mL-1) than lanuginosine (IC50 2.5 mu g mL-1) against HEPG2 in comparison with the standard doxorubacin (IC50 0.27 mu g mL-1). In addition, magnoflorine and lanuginosine exhibited cytotoxicity against U251, with IC50 of 7 and 4 mu g mL-1, respectively. The two compounds were found to be inactive against the Hela cancer cell. On the other hand, the methanol extract showed high antiviral activity against the herpes simplex virus (HSV-1), 76.7% inhibition at 1.1 mu g mL-1, whereas the extract exhibited a moderate antiviral activity against poliovirus type-1 (47% inhibition at the same concentration). This chemical and biological investigation has not been studied previously.