Assignment of human 3-phosphoglycerate dehydrogenase (PHGDH) to human chromosome band 1p12 by fluorescence in situ hybridization.
Assignment of human 3-phosphoglycerate dehydrogenase (PHGDH) to human chromosome band 1p12 by fluorescence in situ hybridization.
复制标题
通过荧光原位杂交将人 3-磷酸甘油酸脱氢酶 (PHGDH) 分配到人染色体带 1p12。
DOI：
10.1159/000015577
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Kim,YH
中科院分区：
文献类型：
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作者：
Baek,JY;Jun,DY;Taub,D;Kim,YH
材料和方法从pha刺激的外周淋巴细胞中制备人类中期细胞，荧光原位杂交（FISH）基本上按照前面所述进行（Shi et al . 1997）。用地高辛dUTP标记人PHGDH基因组克隆探针DNA。用荧光抗地高辛抗体孵育杂交载玻片，然后用DAPI反染，检测特异性杂交信号。为了确认染色体位置，除了使用地高辛标记的人类PHGDH基因组克隆外，还使用地高辛标记的基因组克隆进行了第二次杂交，该基因组克隆先前定位于1q44 （GenomeSystems Inc., St. Louis， MO）。
Materials and methodsHuman metaphase cells were prepared from PHA-stimulated peripheral lymphocytes and fluorescence in situ hybridization (FISH) was essentially performed as described previously (Shi et al" 1997). Probe DNA from human PHGDH genomic clone was labeled with digoxigenin dUTP. Specific hybridization signals were detected by incubating the hybridized slides in fluoresceinated antidigoxigenin antibodies followed by counterstaining with DAPI. In order to confirm chromosome location, a second hybridization was performed utilizing, in addition to the digoxigenin labeled human PHGDH genomic clone, a digoxigenin labeled genomic clone which has been previously localized to 1q44 (GenomeSystems Inc., St. Louis, MO).