Detection and genetic differentiation of human astroviruses: Phylogenetic grouping varies by coding region
Detection and genetic differentiation of human astroviruses: Phylogenetic grouping varies by coding region
复制标题
DOI：
10.1007/s007050050163
复制
发表时间：
1997-01-01
影响因子：
2.7
通讯作者：
Monroe, SS
中科院分区：
文献类型：
--
作者：
Belliot, G;Laveran, H;Monroe, SS
星状病毒是与人类和动物胃肠炎相关的单链、正义RNA病毒。我们描述了一种逆转录-聚合酶链反应（RT-PCR）检测，使用针对非结构蛋白编码区的引物，允许敏感的检测和遗传分型的代表性菌株的7个星状病毒血清型。对参考菌株和几个野生分离株的PCR产物的核苷酸序列进行系统发育分析表明，在开放阅读框架la（ORF la）中存在两个不同的基因组序列。这些基因组与血清型相关：基因组A包括1 - 5型菌株，而基因组B包括6和7型菌株。这种系统发育排列不同于比较ORF Ib或ORF 2的核苷酸序列时所见的血清型的近等距聚类。这是可能的，重组是负责观察到的遗传关系的差异。
Astroviruses are single-stranded, positive-sense RNA viruses that are associated with gastroenteritis in humans and animals. We describe a reverse transcription-polymerase chain reaction (RT-PCR) assay using primers targeted to a nonstructural protein coding region that allowed sensitive detection and genetic typing of representative strains of seven astrovirus serotypes. Phylogenetic analysis of the nucleotide sequences of PCR products from the reference strains and several wild isolates indicated two distinct genogroups of sequences in open reading frame la (ORF la). These genogroups correlated with serotype: genogroup A included strains of types 1 to 5, while genogroup B included strains of types 6 and 7. This phylogenetic arrangement differs from the nearly equidistant clustering of serotypes seen when comparing nucleotide sequences from either ORF Ib or ORF 2. It is possible that recombination was responsible for the observed difference in genetic relationships.