Ultrafast reversible photo-cross-linking reaction: Toward in situ DNA manipulation
Ultrafast reversible photo-cross-linking reaction: Toward in situ DNA manipulation
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DOI：
10.1021/ol801112j
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发表时间：
2008-08-07
期刊：
影响因子：
5.2
通讯作者：
Fujimoto, Kenzo
中科院分区：
文献类型：
--
作者：
Yoshimura, Yoshinaga;Fujimoto, Kenzo
描述了一种新型的3-氰基乙烯基咔唑核苷((CNV)K)的超快可逆DNA链间光交联反应。366 nm光照射1s可使含(CNV)K的寡核苷酸发生光交联，312 nm照射60 S可使光交联的寡核苷酸发生裂解。
We describe a novel ultrafast reversible DNA interstrand photo-cross-linking reaction via 3-cyanovinylcarbazole nucleoside ((CNV)K). Oligodeoxynucleotide (ODN) containing (CNV)K can be photo-cross-linked by irradiation at 366 nm for 1s, and the photo-cross-linked ODN can be split by irradiation at 312 nm for 60 s.