USE OF ACID, ROSE BENGAL, AND STREPTOMYCIN IN THE PLATE METHOD FOR ESTIMATING SOIL FUNGI
USE OF ACID, ROSE BENGAL, AND STREPTOMYCIN IN THE PLATE METHOD FOR ESTIMATING SOIL FUNGI
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DOI：
10.1097/00010694-195003000-00006
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发表时间：
1950-01-01
期刊：
影响因子：
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通讯作者：
MARTIN, JP
中科院分区：
文献类型：
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作者：
MARTIN, JP
用稀释平板法比较了酸、孟加拉玫瑰和链霉素作为细菌生长抑制剂在不同真菌培养基中单独和不同组合使用对土壤真菌数量和种类的影响。连词。1：5000到1：30,000的孟加拉玫瑰阻止了一些土壤中大多数细菌的生长，而另一些土壤中含有足够多的能够在这些浓度下生长的细菌。真菌数量减少了。玫瑰色孟加拉(1：15,000或1：30,000)和30微克/毫升链霉素的组合几乎消除了所有测试土壤中的细菌生长。单用链霉素对117种真菌培养物中的2种有轻微的抑制作用。除了极少数例外，孟加拉玫瑰除了减少蜂群的大小外，几乎没有什么效果。17个真菌在单独含有孟加拉玫瑰或与链霉素混合培养的培养基上生长，在酸性琼脂(pH=4)上不能生长或生长极差。含有1：30,000孟加拉玫瑰花和30微克/毫升链霉素的蛋白胨-葡萄糖琼脂是土壤真菌培养的最佳培养基。与酸化至pH4的相同培养基相比，菌落总数增加了100%，分离到的真菌种类增加了14%。六种真菌一直被分离在相对较大的容器中。在酸性琼脂上很少或从未发现。
A study was made of the comparative use, singly and in various combinations, of acid, rose bengal, and streptomycin as bacterial growth inhibitors in various fungus media for estimating numbers and kinds of soil fungi by the dilution plate method. Concns. of 1:5000 to 1:30,000 of rose bengal prevented growth of most bacteria in some soils, while others contained sufficiently large numbers capable of developing at these concns. that fungus counts were decreased. A combination of rose bengal (1:15,000 or 1:30,000) and 30 ug./ml. of streptomycin virtually eliminated bacterial growth from all soils tested. Streptomycin alone slightly inhibited growth of 2 fungus cultures out of 117 tested. With few exceptions rose bengal had little effect other than reducing the size of the colonies. 17 fungi, which developed on media containing rose bengal alone or in combination with streptomycin, failed to grow or produced extremely poor growth on acidified agar (pH4). Peptone-dextrose agar containing 1:30,000 rose bengal and 30 ug./ml. of streptomycin was rated the best medium for soil-fungus plating. By use of this medium fungus counts were increased as much as 100% and the kinds of fungi isolated by 14% compared with the same medium acidified to pH4. Six fungi that were consistently isolated in relatively large concns. on the new medium were rarely or never found on the acid agar.