T-2 toxin-induced acute skin lesions in Wistar-derived hypotrichotic WBN/ILA-Ht rats
T-2 toxin-induced acute skin lesions in Wistar-derived hypotrichotic WBN/ILA-Ht rats
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DOI：
10.14670/hh-14.337
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发表时间：
1999-04-01
影响因子：
2
通讯作者：
Doi, K
中科院分区：
文献类型：
--
作者：
Albarenque, SM;Shinozuka, J;Doi, K
在治疗后24小时内（24 HAT），对Wistar衍生的低血糖WBN/ILA-Ht大鼠背部皮肤局部应用T-2毒素（10 μ l 0.5 mg/ml溶液至1 cm（2））的急性损伤进行了检查。在表皮中，通过增殖细胞核抗原（PCNA）的免疫染色检测到基底细胞增殖活性在3 HAT的抑郁症，此后PCNA阳性基底细胞的百分比下降。在12 HAT时，除了棘细胞的胞浆内水肿外，基底细胞的嗜酸性变性变得突出，其特征在于具有嗜酸性细胞质和核固缩或核出血的细胞体皱缩。这些细胞核中的大多数对TUNEL呈阳性，TUNEL是一种广泛用于原位检测片段化DNA（即凋亡）的免疫染色，并且此后TUNEL阳性基底细胞的百分比增加。这些基底细胞的细胞核也表现出细胞凋亡的超微结构变化特征。另一方面，在真皮中，包括肥大细胞在内的炎性细胞的浸润在3 HAT时开始，此后增加。此外，毛细血管和小血管内皮变性在GHAT发展，并在此后进展。这些结果表明，T-2毒素直接作用于表皮，并在基底细胞中产生凋亡。
Acute lesions in the dorsal skin topically applied with T-2 toxin (10 mu l of 0.5 mg/ml-solution to 1 cm(2)) were examined in Wistar-derived hypotrichotic WBN/ILA-Ht rats up to 24 hours after treatment (24HAT). In the epidermis, depression of basal cell proliferating activity was detected at 3HAT by immunostaining for proliferating cell nuclear antigen (PCNA), and the percentage of PCNA-positive basal cells decreased thereafter. At 12HAT, in addition to intracytoplasmic edema of spinous cells, acidophilic degeneration of basal cells characterized by shrinkage of cell body with acidophilic cytoplasm and pyknotic or karyorrhectic nuclei became prominent. Most of these nuclei were positive for TUNEL which is a widely used immunostaining for the in situ detection of fragmented DNA, i.e. apoptosis, and the percentage of TUNEL-positive basal cells increased thereafter. The nuclei of these basal cells also showed ultrastructural changes characteristic for apoptosis. On the other hand, in the dermis, infiltration of inflammatory cells including mast cells started at 3HAT and increased thereafter. In addition, capillary and small vessel endothelial degeneration developed at GHAT and progressed thereafter. These results suggest that T-2 toxin directly affects the epidermis and produces apoptosis in basal cells.