Electrochemical detection of 17β-estradiol using DNA aptamer immobilized gold electrode chip
Electrochemical detection of 17β-estradiol using DNA aptamer immobilized gold electrode chip
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DOI：
10.1016/j.bios.2006.10.004
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发表时间：
2007-05-15
影响因子：
12.6
通讯作者：
Gu, Man Bock
中科院分区：
文献类型：
--
作者：
Kim, Yeon Seok;Jung, Ho Sup;Gu, Man Bock
使用DNA适体固定金电极芯片开发了一种用于化学传感的电化学检测方法。通过 SELEX（指数富集配体系统进化）过程从随机 ssDNA 文库中选择特异性结合 17 β-雌二醇的 DNA 适体，该文库由大约 7.2 x 10(14) DNA 分子组成。采用金电极芯片来评估适配体与目标分子之间相互作用产生的电化学信号。 DNA 适体固定在金电极上是基于亲和素-生物素相互作用。测量循环伏安法（CV）和方波伏安法（SWV）值以评估与适体的化学结合。当17β-雌二醇与DNA适体相互作用时，由于结合的17β-雌二醇对亚铁氰化物与铁氰化物之间的氧化还原反应产生的电子流的干扰，电流下降。在阴性对照实验中，由于其他化学物质的存在，电流仅轻微下降。 (c) 2006 Elsevier B.V. 保留所有权利。
An electrochemical detection method for chemical sensing has been developed using a DNA aptamer immobilized gold electrode chip. DNA aptamers specifically binding to 17 beta-estradiol were selected by the SELEX (Systematic Evolution of Ligands by EXponential enrichment) process from a random ssDNA library, composed of approximately 7.2 x 10(14) DNA molecules. Gold electrode chips were employed to evaluate the electrochemical signals generated from interactions between the aptamers and the target molecules. The DNA aptamer immobilization on the gold electrode was based on the avidin-biotin interaction. The cyclic voltametry (CV) and square wave voltametry (SWV) values were measured to evaluate the chemical binding to aptamer. When 17 beta-estradiol interacted with the DNA aptamer, the current decreased due to the interference of bound 17 beta-estradiol with the electron flow produced by a redox reaction between ferrocyanide and ferricyanide. In the negative control experiments, the current decreased only mildly due to the presence of other chemicals. (c) 2006 Elsevier B.V. All rights reserved.