Time-course of changes to nitric oxide signaling pathways in form-deprivation myopia in guinea pigs
Time-course of changes to nitric oxide signaling pathways in form-deprivation myopia in guinea pigs
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DOI：
10.1016/j.brainres.2007.09.077
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发表时间：
2007-12
期刊：
影响因子：
2.9
通讯作者：
Jie Wu;Qiong Liu;X. Yang;Hui Yang;Jun-wen Zeng
中科院分区：
文献类型：
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作者：
Jie Wu;Qiong Liu;X. Yang;Hui Yang;Jun-wen Zeng
本研究旨在观察豚鼠形觉剥夺后不同时间视网膜、脉络膜和巩膜一氧化氮合酶(NOS)活性和环鸟苷酸(CGMP)浓度的时程变化。三组豚鼠分别进行7、14、21天的单眼FD。用放射免疫法测定FD眼和正常眼眼组织中一氧化氮合酶活性和cGMP含量。免疫组织化学方法检测一氧化氮合酶异构体的存在。豚鼠在FD治疗14天后出现相当程度的近视。FD组视网膜一氧化氮合酶活性在发病后7天低于对照组，在发病14天和21天高于对照组。治疗21d后，FD组脉络膜和巩膜NOS活性均高于对照组。FD组视网膜、脉络膜和巩膜组织中cGMP浓度在第21天高于对照组。此外，FD组的视网膜cGMP浓度在第14天也显著高于对照组。我们检测了三种一氧化氮合酶亚型在豚鼠眼组织中的表达。我们的主要观察是近视发展过程中眼后部组织中一氧化氮合酶活性的变化和cGMP浓度的上调。一氧化氮合酶活性升高的作用可能是由cGMP介导的。
The aim of this study was to investigate the time-course change of nitric oxide synthase (NOS) activity and cyclic GMP (cGMP) concentration in the posterior retina, choroid and sclera after differing periods of form-deprivation in guinea pigs. Three groups of guinea pigs were subjected to monocular FD for 7, 14 or 21 days. NOS activity and cGMP concentrations in ocular tissues of FD eyes and control eyes were analyzed by radioimmunoassay. The presence of NOS isoforms was detected by immunohistochemistry. Guinea pigs presented with considerable myopia after 14 days of FD. Retinal NOS activity in the FD group was lower than in the control group after 7 days of FD and was higher than in the control group after 14 and 21 days of FD. The choroidal and scleral NOS activities in the FD groups were higher than in the control groups after 21 days. The cGMP concentrations in the FD groups were higher than in the control groups at 21 days of the retinal, choroidal, and scleral tissues. Furthermore, the retinal cGMP concentration in the FD group was also significantly elevated at 14 days relative to the control group. We detected expression of three NOS isoforms in guinea pig ocular tissues. Our main observations were a change in NOS activity and an up-regulation in cGMP concentrations in posterior ocular tissues during the development of myopia. The function of elevated NOS activity may be mediated by cGMP.