Multi-color miniature dual-axis confocal microscope for point-of-care pathology.
Multi-color miniature dual-axis confocal microscope for point-of-care pathology.
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DOI：
10.1364/ol.37.002430
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发表时间：
2012-06-15
期刊：
影响因子：
3.6
通讯作者：
Liu JT
中科院分区：
文献类型：
--
作者：
Leigh SY;Liu JT
我们提出了一个微型微机电系统（MEMS）为基础的双轴共焦显微镜能够在多个波长的空间共配准荧光成像。该器械具有直径为10 mm的扫描头和直径为2 mm的头端，便于在手术期间引导肿瘤切除。该显微镜具有20 - 200微米的可调焦深，能够在450 × 450微米的视场内以9微米的轴向分辨率和4微米的面内分辨率成像。通过使用一对光栅棱镜组件（GRISMs）来补偿设备远端2 mm直径梯度折射率（GRIN）中继透镜中的色散，实现各个光学部分的同时双色成像。显微镜的轴向响应的实验测量以及反射条目标和新鲜小鼠脑组织的双色图像证明了我们的设备的性能及其在多色体内光学切片显微镜的潜力。
We present a miniature micro-electro-mechanical systems (MEMS)-based dual-axis confocal microscope capable of spatially co-registered fluorescence imaging at multiple wavelengths. This device has a 10-mm diameter scan head with a 2-mm diameter tip for convenient use during surgery to guide tumor resection. The microscope has an adjustable focal depth from 20 – 200 microns and is capable of imaging with an axial resolution of 9 microns and in-plane resolution of 4 microns over a field of view of 450 × 450 microns. Simultaneous two-color imaging of individual optical sections is achieved by using a pair of grating-prism assemblies (GRISMs) to compensate for chromatic dispersion in the 2-mm diameter gradient-index (GRIN) relay lens at the distal tip of the device. Experimental measurements of the axial response of the microscope as well as two-color images of a reflective bar target and fresh mouse brain tissues demonstrate the performance of our device and its potential for multi-color in vivo optical-sectioning microscopy.