Cellular distribution of secretory pathway markers in the haploid synergid cells of Arabidopsis thaliana
Cellular distribution of secretory pathway markers in the haploid synergid cells of Arabidopsis thaliana
复制标题
DOI：
10.1111/tpj.13848
复制
发表时间：
2018-04-01
期刊：
影响因子：
7.2
通讯作者：
Kessler, Sharon A.
中科院分区：
文献类型：
--
作者：
Jones, Daniel S.;Liu, Xunliang;Kessler, Sharon A.
在开花植物中，细胞间的交流在繁殖成功中起着关键作用，因为授粉和受精都需要调节许多不同类型细胞之间的相互作用的途径。其中一些最关键的相互作用是花粉管(PT)和胚囊之间的相互作用，这些相互作用确保了双受精所需的精子细胞的交付。协同细胞的功能是通过分泌小肽来吸引PT，并通过与膜系统和细胞表面相关的膜结合蛋白来接收PT。虽然已经确定了许多调节PT吸引和接收的协同细胞表达的成分，但很少有工具来研究这种细胞类型中膜结合蛋白和内膜系统的成分的定位。在这项研究中，我们描述了七个标记分泌途径组分的荧光标记在拟南芥助细胞中的定位和分布。这些标记被用来研究两个PT受体成分Lorelei和NORTIA的亚细胞分布。Lorelei是一种GPI锚定的表面蛋白，NORTIA是一种抗霉菌基因座O蛋白，两者都存在于助细胞的内膜系统中。这些分泌标记对生殖生物学家和细胞生物学家都是有用的工具，使分析活跃在极地运输中的单倍体细胞内的膜相关运输成为可能。重要说明开花植物繁殖需要调节花粉管吸引和接受的专门细胞之间的细胞间通讯途径的协调。在这项研究中，我们产生了一套标记分泌途径关键成分的荧光标记，并在拟南芥的助细胞中稳定表达，使我们能够详细分析单倍体细胞中细胞运输的调节。
In flowering plants, cell-cell communication plays a key role in reproductive success, as both pollination and fertilization require pathways that regulate interactions between many different cell types. Some of the most critical of these interactions are those between the pollen tube (PT) and the embryo sac, which ensure the delivery of sperm cells required for double fertilization. Synergid cells function to attract the PT through secretion of small peptides and in PT reception via membrane-bound proteins associated with the endomembrane system and the cell surface. While many synergid-expressed components regulating PT attraction and reception have been identified, few tools exist to study the localization of membrane-bound proteins and the components of the endomembrane system in this cell type. In this study, we describe the localization and distribution of seven fluorescent markers that labelled components of the secretory pathway in synergid cells of Arabidopsis thaliana. These markers were used in co-localization experiments to investigate the subcellular distribution of the two PT reception components LORELEI, a GPI-anchored surface protein, and NORTIA, a MILDEW RESISTANCE LOCUS O protein, both found within the endomembrane system of the synergid cell. These secretory markers are useful tools for both reproductive and cell biologists, enabling the analysis of membrane-associated trafficking within a haploid cell actively involved in polar transport.Significance Statement Flowering plant reproduction requires the coordination of intercellular communication pathways between specialized cells regulating pollen tube attraction and reception. In this study, we generated a suite of fluorescent markers labeling key components of the secretory pathway and stably expressed them in the synergid cells of Arabidopsis thaliana, enabling the detailed analysis of the regulation of cellular trafficking in a haploid cell.