The baculovirus P10 protein of Autographa californica qucleopolyhedrovirus forms two distinct cytoskeletal-like structures and associates with polyhedral occlusion bodies during infection
The baculovirus P10 protein of Autographa californica qucleopolyhedrovirus forms two distinct cytoskeletal-like structures and associates with polyhedral occlusion bodies during infection
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DOI：
10.1016/j.virol.2007.09.043
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发表时间：
2008-02-20
期刊：
影响因子：
3.7
通讯作者：
King, Linda A.
中科院分区：
文献类型：
--
作者：
Carpentier, David C. J.;Griffiths, Caroline M.;King, Linda A.
杆状病毒微管相关P10蛋白的作用尚不清楚。P10以前被认为与细胞核和细胞质中的许多胞浆样或胞浆相关结构的形成有关，被认为参与病毒多面体包涵体的形态发生。通过免疫荧光激光扫描共聚焦显微镜在TN368细胞中研究了这些结构的形成，TN368细胞是一种适合研究病毒与宿主细胞细胞骨架相互作用的模型系统。我们发现，苜蓿银纹夜蛾核型多角体病毒P10蛋白形成两个不同的细胞质样结构，微管相关的丝和核周管状聚集体。P10也与多面体咬合体有关。用秋水仙素解聚微管网络可防止P10细丝的形成，但不能防止P10小管的形成。秋水仙素处理增强了P10与闭塞体的关联。P10的瞬时表达表明，在没有其他病毒蛋白的情况下，细丝和小管都可以形成。我们推测了P10蛋白在病毒感染和形态发生过程中的一些可能作用。(C)2007年爱思唯尔公司All rights reserved.
The role of the microtubule-associated P 10 protein of baculoviruses is not yet understood. P 10 has previously been linked with the formation of a number of cytoskeletal-like or cytoskeleton-associated structures in the nucleus and cytoplasm, thought to be involved in the morphogenesis of virus polyhedral occlusion bodies. The formation of these structures was studied by immunofluorescence laser scanning confocal microscopy in TN368 cells, a model system amenable to the study of virus interaction with the host cell cytoskeleton. We show that the Autographa californica nucleopolyhedrovirus P10 protein forms two distinct cytoskeletal-like structures, microtubule-associated filaments and perinuclear tubular aggregates. P10 also associates with polyhedral occlusion bodies. Depolymerisation of the microtubule network with colchicine prevents formation of P10 filaments but not of P10 tubules. Colchicine treatment enhances the association of P10 with occlusion bodies. Transient expression of P 10 showed that both filaments and tubules can form in the absence of other viral proteins. We postulate a number of possible roles of the P10 protein during virus infection and morphogenesis. (C) 2007 Elsevier Inc. All rights reserved.