Serum cholesterol promotes the growth of Candida glabrata in the presence of fluconazole
Serum cholesterol promotes the growth of Candida glabrata in the presence of fluconazole
复制标题
氟康唑存在下血清胆固醇促进光滑念珠菌生长
DOI：
10.1007/s10156-012-0531-3
复制
发表时间：
2013
影响因子：
2.2
通讯作者：
Miyazaki Y
中科院分区：
文献类型：
--
作者：
Nagi M;Tanabe K;Nakayama H;Yamagoe S;Umeyama T;Oura T;Ohno H;Kajiwara S;Miyazaki Y
病原真菌光滑念珠菌被认为在感染过程中利用细胞外甾醇，但关于该真菌的甾醇摄取机制的报道很少。添加血清促进了C的生长。光滑细胞在存在甾醇抑制剂氟康唑的情况下，可能是从血清中掺入胆固醇的结果。我们证明，大部分胆固醇被消除的脂蛋白缺乏血清不能挽救氟康唑处理的C的生长。 glabratacells，但它成功地促进了甾醇转运蛋白基因AUS1的表达。在向脂蛋白缺乏的血清中补充游离胆固醇后，该血清再次能够促进氟康唑处理的C的生长。光滑。在非病原性酿酒酵母中观察到血清介导的氟康唑抑制生长救援，随后是厌氧甾醇摄取的激活。这些结果表明，当甾醇摄取被激活时，血清胆固醇被掺入酵母细胞中以补偿甾醇的消耗。血清胆固醇的摄取可以支持C的生长。血流感染期间的光滑细胞。
The pathogenic fungusCandida glabratais thought to utilize extracellular sterols during infection, but there have been few reports on the sterol uptake mechanisms of this fungus. The addition of serum promoted the growth ofC. glabratacells in the presence of the sterol inhibitor fluconazole, probably as the result of incorporation of cholesterol from serum. We demonstrated that lipoprotein-deficient serum, in which most of the cholesterol was eliminated, could not rescue the growth of fluconazole-treatedC. glabratacells, but it successfully promoted the expression of the sterol transporter geneAUS1. After supplementation of free cholesterol to lipoprotein-deficient serum, the serum was again competent to promote the growth of fluconazole-treatedC. glabrata. The serum-mediated growth rescue from fluconazole inhibition was observed in the nonpathogenic yeastSaccharomyces cerevisiaewhen it was followed by the activation of anaerobic sterol uptake. These results suggested that serum cholesterol was incorporated into yeast cells to compensate for sterol depletion when sterol uptake was activated. The uptake of serum cholesterol could support the growth ofC. glabratacells during bloodstream infections.